AMAb90662

antibody from Atlas Antibodies

Targeting: ANLN ANILLIN, scra, Scraps

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AMAb90662

Provider

Atlas Antibodies

Proper citation

Product name

Anti-ANLN

Antibody type

Monoclonal

Description

Monoclonal Antibody against Human ANLN, Clone ID: CL0303, Gene description: anillin, actin binding protein, Alternative Gene Names: ANILLIN, scra, Scraps, Validated applications: WB, IHC, ICC, Uniprot ID: Q9NQW6, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

CL0303

Vial size

100 µl

Concentration

1.0 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

Deep Visual Proteomics defines single-cell identity and heterogeneity.

Mund A, Coscia F, Kriston A, Hollandi R, Kovács F, Brunner AD, Migh E, Schweizer L, Santos A, Bzorek M, Naimy S, Rahbek-Gjerdrum LM, Dyring-Andersen B, Bulkescher J, Lukas C, Eckert MA, Lengyel E, Gnann C, Lundberg E, Horvath P, Mann M
Nature biotechnology 2022 Aug;40(8):1231-1240

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Anillin is required for tumor growth and regulated by miR-15a/miR-16-1 in HBV-related hepatocellular carcinoma

Lian Y, Huang Y, Wang J, Deng M, Xia T, Zeng M, Chen M, Wang H, Huang Y
Aging 2018;10(8):1884-1901

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Regulation of actin-binding protein ANLN by antitumor miR-217 inhibits cancer cell aggressiveness in pancreatic ductal adenocarcinoma.

Idichi T, Seki N, Kurahara H, Yonemori K, Osako Y, Arai T, Okato A, Kita Y, Arigami T, Mataki Y, Kijima Y, Maemura K, Natsugoe S
Oncotarget 2017 Aug 8;8(32):53180-53193

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ANLN antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Sample type

Human

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining in HeLa cell line with Anti-ANLN monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Sample type

Human

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human testis and skeletal muscle tissues using AMAb90662 antibody. Corresponding ANLN RNA-seq data are presented for the same tissues.

Sample type

Human

Protocol

Protocol

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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