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Western blot
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- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- HPA005680 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA005680, RRID:AB_2667388
- Product name
- Anti-ANLN
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human ANLN, Gene description: anillin, actin binding protein, Alternative Gene Names: ANILLIN, scra, Scraps, Validated applications: WB, IHC, Uniprot ID: Q9NQW6, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.2 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references A Novel Gene Expression Scoring System Predicts Recurrence in Non-Muscle-Invasive Bladder Cancer Patients.
Drak/STK17A Drives Neoplastic Glial Proliferation through Modulation of MRLC Signaling
ANLN is a prognostic biomarker independent of Ki-67 and essential for cell cycle progression in primary breast cancer.
Systematic antibody generation and validation via tissue microarray technology leading to identification of a novel protein prognostic panel in breast cancer
Generation of monospecific antibodies based on affinity capture of polyclonal antibodies
Kayama E, Uemura M, Onagi A, Meguro S, Ogawa S, Yaginuma K, Matsuoka K, Hoshi S, Koguchi T, Hata J, Sato Y, Akaihata H, Honma R, Watanabe S, Kojima Y
Cancer medicine 2024 Nov;13(22):e70349
Cancer medicine 2024 Nov;13(22):e70349
Konstantopoulos K, Tran A, Wisniewski E, Mistriotis P, Stoletov K, Parlani M, Amitrano A, Ifemembi B, Lee S, Bera K, Zhang Y, Tuntithavornwat S, Afthinos A, Kiepas A, Jamieson J, Zuo Y, Habib D, Wu P, Martin S, Gerecht S, Gu L, Lewis J, Kalab P, Friedl P
2023
2023
Drak/STK17A Drives Neoplastic Glial Proliferation through Modulation of MRLC Signaling
Chen A, Wardwell-Ozgo J, Shah N, Wright D, Appin C, Vigneswaran K, Brat D, Kornblum H, Read R
Cancer Research 2019;79(6):1085-1097
Cancer Research 2019;79(6):1085-1097
ANLN is a prognostic biomarker independent of Ki-67 and essential for cell cycle progression in primary breast cancer.
Magnusson K, Gremel G, Rydén L, Pontén V, Uhlén M, Dimberg A, Jirström K, Pontén F
BMC cancer 2016 Nov 18;16(1):904
BMC cancer 2016 Nov 18;16(1):904
Systematic antibody generation and validation via tissue microarray technology leading to identification of a novel protein prognostic panel in breast cancer
O´Leary P, Penny S, Dolan R, Kelly C, Madden S, Rexhepaj E, Brennan D, McCann A, Pontén F, Uhlén M, Zagozdzon R, Duffy M, Kell M, Jirström K, Gallagher W
BMC Cancer 2013;13(1)
BMC Cancer 2013;13(1)
Generation of monospecific antibodies based on affinity capture of polyclonal antibodies
Hjelm B, Forsström B, Igel U, Johannesson H, Stadler C, Lundberg E, Ponten F, Sjöberg A, Rockberg J, Schwenk J, Nilsson P, Johansson C, Uhlén M
Protein Science 2011;20(11):1824-1835
Protein Science 2011;20(11):1824-1835
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ANLN antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human testis and skeletal muscle tissues using HPA005680 antibody. Corresponding ANLN RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
