Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [7]
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- Product number
- NBP2-38630 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Anillin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Anillin antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Anillin Antibody [NBP2-38630] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Human cell line RT-4 Lane 3: Human cell line U-251 MG
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Anillin Antibody [NBP2-38630] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-ANLN antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-38630] - Staining of human testis shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-38630] - Staining of human skeletal muscle shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-38630] - Staining of human colon shows moderate to strong nuclear positivity in a subset of glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-38630] - Analysis in human testis and skeletal muscle tissues. Corresponding ANLN RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-38630] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-38630] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-38630] - Staining of human placenta shows moderate to strong nuclear positivity in a subset of trophoblastic cells.