PAB29430

antibody from Abnova Corporation

Targeting: ANLN ANILLIN, scra, Scraps

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29430

Provider

Abnova Corporation

Product name

ANLN polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human ANLN.

Antigen sequence

DLFSDVLEEGELDMEKSQEEMDQALAESSEEQEDA
LNISSMSLLAPLAQTVGVVSPESLVSTPRLELKDT
SRSDESPKPGKFQRTRVPRAESGDSLGSEDRDLLY
SIDAYRSQRFKETERPSIKQVIVRKEDV


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Human cell line RT-4 Lane 2: Human cell line U-251MG with ANLN polyclonal antibody (Cat # PAB29430) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with ANLN polyclonal antibody (Cat # PAB29430) at 1-4 ug/mL concentration shows positivity in nucleus but excluded from the nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human testis with ANLN polyclonal antibody (Cat # PAB29430) shows moderate nuclear positivity in cells in seminiferous ducts at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)