Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [6]
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Validation data
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- Product number
- NBP2-52908 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Anillin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Specificity of human Anillin antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
LRGRFDKGNIWSAEK
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Anillin Antibody (CL0303) [NBP2-52908] - Lane 1: Marker [kDa] Lane 2:Human cell line U-251 MG
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Anillin Antibody (CL0303) [NBP2-52908] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ANLN antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Anillin Antibody (CL0303) [NBP2-52908] - Interaction of Anillin with GST-fused protein: MDA-MB-231 cells were lysed in TBS, pH 7.5, containing 0.75% Triton X-100 with inhibitors of proteases and phosphatases. Lysate was sonicated and cleared by centrifugation. for the input, sample buffer was added to part of the lysate and heated fro 10min 95 C. The rest of the lysate was incubated with either GST or GST-fused protein immobilized on agarose beads for 2h in 4 C. the beads were washed three times in lysis buffer, resuspended in sample buffer and heated for 10min 95 C. The samples were resolved using SDS-PAGE and immunoblotted with anti-ANLN antibody. This image was submitted by customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908] - Staining of human colorectal cancer shows moderate to strong nuclear positivity in a subset of tumor cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908] - Staining of human testis shows moderate to strong nuclear positivity in a subset of cells in seminiferous ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908] - Staining of human rectum shows moderate to strong nuclear positivity in a subset of glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908] - Staining of human skin shows moderate to strong nuclear positivity in a subset of dermal cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908] - Staining of human skeletal muscle shows no nuclear positivity in striated muscle fibers as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908] - Staining in human testis and skeletal muscle tissues. Corresponding ANLN RNA-seq data are presented for the same tissues.