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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Flow cytometry [2]
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- Product number
- RQ4636 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Anillin Antibody / Scraps
- Antibody type
- Polyclonal
- Description
- This highly specific Anillin antibody is suitable for use in Western blot/Immunofluorescence/Flow cytometry applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Anillin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) placenta, 2) SW620, 3) A431, 4) HL60, 5) PC-3 and 6) mouse NIH 3T3 lysate with Anillin antibody at 0.5ug/ml. Predicted molecular weight ~124 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HeLa and 2) 293T cell lysate with Anillin antibody at 0.5ug/ml. Predicted molecular weight ~124 kDa but may be observed at higher molecular weights due to glycosylation.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HeLa, 2) human MCF7, 3) rat PC-12 and 4) mouse NIH 3T3 cell lysate with Anillin antibody. Predicted molecular weight ~124 kDa but may be observed at higher molecular weights due to glycosylation.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human U-2 OS cells with Anillin antibody (green) and Alpha Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human A431 cells with Anillin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunoprecipitation of Anillin protein from 500ug of human U-251 whole cell lysate with 2ug of Anillin antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human U-2 OS cells with Anillin antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Anillin antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human U-251 cells with Anillin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Anillin antibody.
