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- Antibody Data
- Antigen structure
- References [13]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- 25443-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#25443-1-AP, RRID:AB_2750836
- Product name
- TTC35 antibody
- Antibody type
- Polyclonal
- Description
- TTC35 antibody (Cat. #25443-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Role of a holo-insertase complex in the biogenesis of biophysically diverse ER membrane proteins.
A selectivity filter in the ER membrane protein complex limits protein misinsertion at the ER.
Mechanism of an intramembrane chaperone for multipass membrane proteins.
WNK kinases sense molecular crowding and rescue cell volume via phase separation.
ATP13A1 prevents ERAD of folding-competent mislocalized and misoriented proteins.
A specific EMC subunit supports Dengue virus infection by promoting virus membrane fusion essential for cytosolic genome delivery.
The endoplasmic reticulum membrane complex promotes proteostasis of GABA(A) receptors.
WNK1 is an assembly factor for the human ER membrane protein complex.
Structural and mechanistic basis of the EMC-dependent biogenesis of distinct transmembrane clients.
The ER membrane protein complex is required to ensure correct topology and stable expression of flavivirus polyproteins.
The ER membrane protein complex promotes biogenesis of sterol-related enzymes maintaining cholesterol homeostasis.
EMC Is Required to Initiate Accurate Membrane Protein Topogenesis.
SLC25A46 is required for mitochondrial lipid homeostasis and cristae maintenance and is responsible for Leigh syndrome.
Page KR, Nguyen VN, Pleiner T, Tomaleri GP, Wang ML, Guna A, Wang TY, Chou TF, Voorhees RM
bioRxiv : the preprint server for biology 2023 Nov 29;
bioRxiv : the preprint server for biology 2023 Nov 29;
A selectivity filter in the ER membrane protein complex limits protein misinsertion at the ER.
Pleiner T, Hazu M, Pinton Tomaleri G, Nguyen VN, Januszyk K, Voorhees RM
The Journal of cell biology 2023 Aug 7;222(8)
The Journal of cell biology 2023 Aug 7;222(8)
Mechanism of an intramembrane chaperone for multipass membrane proteins.
Smalinskaitė L, Kim MK, Lewis AJO, Keenan RJ, Hegde RS
Nature 2022 Nov;611(7934):161-166
Nature 2022 Nov;611(7934):161-166
WNK kinases sense molecular crowding and rescue cell volume via phase separation.
Boyd-Shiwarski CR, Shiwarski DJ, Griffiths SE, Beacham RT, Norrell L, Morrison DE, Wang J, Mann J, Tennant W, Anderson EN, Franks J, Calderon M, Connolly KA, Cheema MU, Weaver CJ, Nkashama LJ, Weckerly CC, Querry KE, Pandey UB, Donnelly CJ, Sun D, Rodan AR, Subramanya AR
Cell 2022 Nov 23;185(24):4488-4506.e20
Cell 2022 Nov 23;185(24):4488-4506.e20
ATP13A1 prevents ERAD of folding-competent mislocalized and misoriented proteins.
McKenna MJ, Adams BM, Chu V, Paulo JA, Shao S
Molecular cell 2022 Nov 17;82(22):4277-4289.e10
Molecular cell 2022 Nov 17;82(22):4277-4289.e10
A specific EMC subunit supports Dengue virus infection by promoting virus membrane fusion essential for cytosolic genome delivery.
Bagchi P, Speckhart K, Kennedy A, Tai AW, Tsai B
PLoS pathogens 2022 Jul;18(7):e1010717
PLoS pathogens 2022 Jul;18(7):e1010717
The endoplasmic reticulum membrane complex promotes proteostasis of GABA(A) receptors.
Whittsette AL, Wang YJ, Mu TW
iScience 2022 Aug 19;25(8):104754
iScience 2022 Aug 19;25(8):104754
WNK1 is an assembly factor for the human ER membrane protein complex.
Pleiner T, Hazu M, Tomaleri GP, Januszyk K, Oania RS, Sweredoski MJ, Moradian A, Guna A, Voorhees RM
Molecular cell 2021 Jul 1;81(13):2693-2704.e12
Molecular cell 2021 Jul 1;81(13):2693-2704.e12
Structural and mechanistic basis of the EMC-dependent biogenesis of distinct transmembrane clients.
Miller-Vedam LE, Bräuning B, Popova KD, Schirle Oakdale NT, Bonnar JL, Prabu JR, Boydston EA, Sevillano N, Shurtleff MJ, Stroud RM, Craik CS, Schulman BA, Frost A, Weissman JS
eLife 2020 Nov 25;9
eLife 2020 Nov 25;9
The ER membrane protein complex is required to ensure correct topology and stable expression of flavivirus polyproteins.
Ngo AM, Shurtleff MJ, Popova KD, Kulsuptrakul J, Weissman JS, Puschnik AS
eLife 2019 Sep 13;8
eLife 2019 Sep 13;8
The ER membrane protein complex promotes biogenesis of sterol-related enzymes maintaining cholesterol homeostasis.
Volkmar N, Thezenas ML, Louie SM, Juszkiewicz S, Nomura DK, Hegde RS, Kessler BM, Christianson JC
Journal of cell science 2019 Jan 16;132(2)
Journal of cell science 2019 Jan 16;132(2)
EMC Is Required to Initiate Accurate Membrane Protein Topogenesis.
Chitwood PJ, Juszkiewicz S, Guna A, Shao S, Hegde RS
Cell 2018 Nov 29;175(6):1507-1519.e16
Cell 2018 Nov 29;175(6):1507-1519.e16
SLC25A46 is required for mitochondrial lipid homeostasis and cristae maintenance and is responsible for Leigh syndrome.
Janer A, Prudent J, Paupe V, Fahiminiya S, Majewski J, Sgarioto N, Des Rosiers C, Forest A, Lin ZY, Gingras AC, Mitchell G, McBride HM, Shoubridge EA
EMBO molecular medicine 2016 Sep;8(9):1019-38
EMBO molecular medicine 2016 Sep;8(9):1019-38
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 25443-1-AP( TTC35 Antibody) at dilution of 1:600
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The TTC35 antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human TTC35. This antibody recognizes human, mouse, rat antigen. The TTC35 antibody has been validated for the following applications: ELISA, WB, IP analysis.
