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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-59248 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NEK8 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LSTERELFAW GRGDSGRLGL GTRESHSCPQ QVPMPPGQEA QRVVCGIDSS MILTVPGQAL ACGSNRFNKL G
- Concentration
- 0.3 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NEK8 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a NEK8 Polyclonal Antibody (Product # PA5-59248).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NEK8 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a NEK8 Polyclonal Antibody (Product # PA5-59248).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of NEK8 in human cell line U-251 MG shows positivity in microtubules & nucleus but excluded from the nucleoli. Samples were probed using a NEK8 Polyclonal Antibody (Product # PA5-59248).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NEK8 in human gallbladder using a NEK8 Polyclonal Antibody (Product # PA5-59248) shows strong cytoplasmic positivity in glandular cells.
