Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-116501 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NEK8 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Positive Samples: HeLa, Jurkat, K-562, Mouse liver Immunogen sequence: GSVRMRRAEK SVAPSNTGSR TTSVRCRGIP RGPVRPAIPP PLSSVYAWGG GLGTPLRLPM LNTEVVQVAA GRTQKAGVTR SGRLILWEAP PLGAGGGSLL PGAVEQPQPQ FISRFLEGQS G
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 8.68 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NEK8 in extracts of various cell lines. Samples were incubated with NEK8 Polyclonal antibody (Product # PA5-116501) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of NEK8 in HeLa cells using NEK8 Polyclonal Antibody (Product # PA5-116501) at a dilution of 1:100. Blue: DAPI for nuclear staining.
