Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-61801 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CBX7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: ADADLAEGPP PWTPALPSSE VTVTDITANS ITVTFREAQA AEGFFRDRSG KF Highest antigen sequence identity to the following orthologs: Mouse - 85%, Rat - 88%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Bioinformatic analysis of the expression and prognostic value of chromobox family proteins in human breast cancer.
Li X, Gou J, Li H, Yang X
Scientific reports 2020 Oct 20;10(1):17739
Scientific reports 2020 Oct 20;10(1):17739
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CBX7 Polyclonal Antibody (Product # PA5-61801) and a 28kDa band corresponding to CBX7 was observed across cell lines tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of U-2 OS (Lane 1), LNCaP (Lane 2), A-431 (Lane 3), Hep G2 (Lane 4), HeLa (Lane 5), COLO 205 (Lane 6) and HCT 116 (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CBX7 in human kidney tissue shows strong cytoplasmic and nuclear positivity in cells in tubules. Samples were probed using a CBX7 Polyclonal Antibody (Product # PA5-61801).