LS-C682419

antibody from LSBio

Targeting: CBX7

Western blot (Enhanced validation)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C682419

Provider

LSBio

Product name

CBX7 Antibody (clone 3H9D7) LS-C682419

Antibody type

Monoclonal

Description

Purified

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

3H9D7

Storage

Short term: store at 4°C. Long term: store at -20°C.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis using CBX7 mAb against HEK293 (1) and CBX7 (AA: 15-147)-hIgGFc transfected HEK293 (2) cell lysate.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis using CBX7 mAb against human CBX7 (AA: 15-147) recombinant protein. (Expected MW is 41.3 kDa)

ELISA

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunofluorescence analysis of Hela cells using CBX7 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunofluorescence analysis of Hela cells using CBX7 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunofluorescence analysis of Hela cells using CBX7 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunofluorescence analysis of Hela cells using CBX7 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunofluorescence analysis of Hela cells using CBX7 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.