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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Western blot [2]
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- Product number
- HPA014909 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA014909, RRID:AB_1859055
- Product name
- Anti-ZFPL1
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human ZFPL1, Gene description: zinc finger protein-like 1, Alternative Gene Names: D11S750, MCG4, Validated applications: WB, IHC, ICC, Uniprot ID: O95159, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.2 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Tetherin antagonism by SARS‐CoV‐2 ORF3a and spike protein enhances virus release
In vivo identification of GTPase interactors by mitochondrial relocalization and proximity biotinylation
The WDR11 complex facilitates the tethering of AP-1-derived vesicles
The golgin coiled-coil proteins capture different types of transport carriers via distinct N-terminal motifs
The specificity of vesicle traffic to the Golgi is encoded in the golgin coiled-coil proteins
Stewart H, Palmulli R, Johansen K, McGovern N, Shehata O, Carnell G, Jackson H, Lee J, Brown J, Burgoyne T, Heeney J, Okkenhaug K, Firth A, Peden A, Edgar J
EMBO reports 2023;24(12)
EMBO reports 2023;24(12)
Stewart H, Palmulli R, Johansen K, McGovern N, Shehata O, Carnell G, Jackson H, Lee J, Brown J, Burgoyne T, Heeney J, Okkenhaug K, Firth A, Peden A, Edgar J
2022
2022
In vivo identification of GTPase interactors by mitochondrial relocalization and proximity biotinylation
Gillingham A, Bertram J, Begum F, Munro S
eLife 2019;8
eLife 2019;8
The WDR11 complex facilitates the tethering of AP-1-derived vesicles
Navarro Negredo P, Edgar J, Manna P, Antrobus R, Robinson M
Nature Communications 2018;9(1)
Nature Communications 2018;9(1)
The golgin coiled-coil proteins capture different types of transport carriers via distinct N-terminal motifs
Wong M, Gillingham A, Munro S
BMC Biology 2017;15(1)
BMC Biology 2017;15(1)
The specificity of vesicle traffic to the Golgi is encoded in the golgin coiled-coil proteins
Wong M, Munro S
Science 2014;346(6209)
Science 2014;346(6209)
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Enhanced validation
Enhanced validation
- Submitted by
- klas2
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of cell lysate from U-2 OS cells transfected with either siRNA targeting ZFPL1 or control siRNA. Lane 1: Marker (250, 130, 95, 72, 55, 36, 28, 17, 10) Lane 2: Cell lysate from U-2OS cells transfected with siRNA targeting ZFPL1 Lane 3: N/A Lane 4: Cell lysate from U-2OS cells transfected with control siRNA Right image, lane 1-4: loading control
- Sample type
- U-2 OS
- Primary Ab dilution
- 1:258
- Conjugate
- Horseradish Peroxidase
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1:3000
- Knockdown/Genetic Approaches Application
- Western blot
Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ZFPL1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Sample type
- Human
- Protocol
- Protocol
