Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 12226-1-AP - Provider product page
- Provider
- Proteintech Group
- Product name
- SH2B1 antibody
- Antibody type
- Polyclonal
- Description
- SH2B1 antibody (Cat. #12226-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Neuronal SH2B1 attenuates apoptosis in an MPTP mouse model of Parkinson's disease via promoting PLIN4 degradation.
Han X, Liu Y, Dai Y, Xu T, Hu Q, Yi X, Rui L, Hu G, Hu J
Redox biology 2022 Jun;52:102308
Redox biology 2022 Jun;52:102308
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 12226-1-AP( SH2B1 Antibody) at dilution of 1:700
- Validation comment
- WB
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using 12226-1-AP( SH2B1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
- Validation comment
- IF
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-SH2B1 (IP:12226-1-AP, 4ug; Detection:12226-1-AP 1:600) with HEK-293 cells lysate 3000ug.
- Validation comment
- IP
Supportive validation
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human ovary tissue slide using 12226-1-AP( SH2B1 Antibody) at dilution of 1:50 (under 10x lens)
- Validation comment
- IHC
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human ovary tissue slide using 12226-1-AP( SH2B1 Antibody) at dilution of 1:50 (under 40x lens)
- Validation comment
- IHC