15548-1-AP

antibody from Proteintech Group

Targeting: NADK FLJ13052

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

15548-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#15548-1-AP, RRID:AB_2147345

Product name

NADK antibody

Antibody type

Polyclonal

Description

NADK antibody (Cat. #15548-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

G-protein-coupled estrogen receptor 1 promotes peritoneal metastasis of gastric cancer through nicotinamide adenine dinucleotide kinase 1-mediated redox modulation.

Wu T, Ding K, Wang C, Lin G, Xie C, Chen X, Li Q, Yu F, Mao Y, Hong W, Lu L, Li S
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2024 Feb 15;38(3):e23449

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Nicotinamide adenine dinucleotide kinase promotes lymph node metastasis of NSCLC via activating ID1 expression through BMP pathway.

Zeng Z, Gao J, Chen T, Zhang Z, Li M, Fan Q, Liu G, Li X, Li Z, Zhong C, Yao F, Sun L, Deng Y, Li M
International journal of biological sciences 2023;19(10):3184-3199

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NADK-mediated de novo NADP(H) synthesis is a metabolic adaptation essential for breast cancer metastasis.

Ilter D, Drapela S, Schild T, Ward NP, Adhikari E, Low V, Asara J, Oskarsson T, Lau EK, DeNicola GM, McReynolds MR, Gomes AP
Redox biology 2023 May;61:102627

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G6PD-mediated increase in de novo NADP(+) biosynthesis promotes antioxidant defense and tumor metastasis.

Zhang Y, Xu Y, Lu W, Li J, Yu S, Brown EJ, Stanger BZ, Rabinowitz JD, Yang X
Science advances 2022 Jul 22;8(29):eabo0404

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Glucose-6-phosphate dehydrogenase exerts antistress effects independently of its enzymatic activity.

Jin X, Li X, Li L, Zhong B, Hong Y, Niu J, Li B
The Journal of biological chemistry 2022 Dec;298(12):102587

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Upregulation of Antioxidant Capacity and Nucleotide Precursor Availability Suffices for Oncogenic Transformation.

Zhang Y, Xu Y, Lu W, Ghergurovich JM, Guo L, Blair IA, Rabinowitz JD, Yang X
Cell metabolism 2021 Jan 5;33(1):94-109.e8

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Human DNA ligase IV is able to use NAD+ as an alternative adenylation donor for DNA ends ligation.

Chen SH, Yu X
Nucleic acids research 2019 Feb 20;47(3):1321-1334

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NADP(+) is an endogenous PARP inhibitor in DNA damage response and tumor suppression.

Bian C, Zhang C, Luo T, Vyas A, Chen SH, Liu C, Kassab MA, Yang Y, Kong M, Yu X
Nature communications 2019 Feb 11;10(1):693

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

RAW264.7 cells were subjected to SDS PAGE followed by western blot with 15548-1-AP(NADK antibody) at dilution of 1:1000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using NADK antibody 15548-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human kidney using 15548-1-AP(NADK antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human kidney using 15548-1-AP(NADK antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue