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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AF6149 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse/Rat Draxin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse and rat Draxin in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human Draxin is observed.
- Reactivity
- Mouse, Rat
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q6PAL1- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Rat Draxin by Western Blot. Western blot shows lysates of rat embryonic hippocampal glial cells. PVDF Membrane was probed with 1 µg/mL of Mouse/Rat Draxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6149) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Draxin at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Draxin in Mouse Embryo. Draxin was detected in immersion fixed frozen sections of mouse embryo (E13) using Mouse Draxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6149) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cells in the midbrain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
