11085-1-AP

antibody from Proteintech Group

Targeting: PARD3 ASIP, Baz, Bazooka, PAR3, PARD3A, PPP1R118

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

11085-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#11085-1-AP, RRID:AB_2159649

Product name

PARD3 antibody

Antibody type

Polyclonal

Description

KD/KO validated PARD3 antibody (Cat. #11085-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Ameloblastin Amphipathic Helix Motif mediates Ameloblast Polarization and Prismatic Enamel Formation via a RhoA Signaling Pathway.

Visakan G, Bapat RA, Cai J, Suwandi ET, Joester D, Kegulian NC, Sarkisians E, Aghazadeh M, Webster S, Moradian-Oldak J
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Foxq1-Wnt5a Axis Activation in Dental Papilla Stem Cells Promotes Odontogenesis on Acellular Matrix: A Laboratory Investigation.

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International endodontic journal 2026 Apr 8;

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NDR1/2 kinases regulate cell polarization and cell motility through Cdc42 GTPase and Pard3 signaling in mammalian cells.

Gu J, Marjanovic J, Tomic-Canic M, Zhang F, Verde F
bioRxiv : the preprint server for biology 2025 Oct 31;

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Single-cell RNA sequencing and cell-cell communication analysis reveal tumor microenvironment associated with chemotherapy responsiveness in ovarian cancer.

Jiang X, Chen N, Wei Q, Luo X, Liu X, Xie L, Yi P, Xu J
Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico 2025 Mar;27(3):1000-1012

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Discovery and Functional Characterization of a Recombinant Fragment of Human Collagen Type XVII.

Piao L, Li J, Li X, Su Y, Yuan X, Chang S, Cheng X, Fu S, Kong R
Journal of agricultural and food chemistry 2025 Mar 19;73(11):6724-6735

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C1QL1 regulates auditory nerve fibers growth via ELMO1-DOCK180-RAC1 integrin.

Chen M, Qi Y, Gong S
Acta oto-laryngologica 2025 Jun;145(6):476-482

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CCL2/CCR4 participates in mast cell-mediated epithelial mesenchymal transition in endometriosis.

Ding S, Mao X, Zhu L, Xu X, Chen S, Zou G, Xu P, Wang J, Zhang X
Reproductive biomedicine online 2025 Aug;51(2):104796

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Decoding the pathogenesis of spermatogenic failure in cryptorchidism through single-cell transcriptomic profiling.

Wang X, Liu Q, Zhuang Z, Cheng J, Zhang W, Jiang Q, Guo Y, Li R, Lu X, Cui L, Weng J, Tang Y, Yue J, Gao S, Hong K, Qiao J, Jiang H, Guo J, Zhang Z
Cell reports. Medicine 2024 Sep 17;5(9):101709

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PARD3 drives tumorigenesis through activating Sonic Hedgehog signalling in tumour-initiating cells in liver cancer.

Wu J, Tan HY, Chan YT, Lu Y, Feng Z, Yuan H, Zhang C, Feng Y, Wang N
Journal of experimental & clinical cancer research : CR 2024 Feb 6;43(1):42

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Nox1-based NADPH oxidase regulates the Par protein complex activity to control cell polarization.

Valdivia A, Duran C, Lee M, Williams HC, Lee MY, San Martin A
Frontiers in cell and developmental biology 2023;11:1231489

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Establishment and identification of cardiomyocyte arhGEF18 gene conditional knockout mice.

Wan K, Yuan W, Lv T
Pediatric discovery 2023 Sep;1(2):e20

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Colonic mechanism of serum NAD(+) depletion induced by DEHP during pregnancy.

Hong Y, Ning X, Liang YY, Li XL, Cui Y, Wu W, Cai Y, Zhao S, Zhu M, Zhong TX, Wang H, Xu DX, Xu T, Zhao LL
The Science of the total environment 2023 May 10;872:162188

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Glucocorticoid receptor-mediated Nr1d1 chromatin circadian misalignment in stress-induced irritable bowel syndrome.

Zheng G, Pang S, Wang J, Wang F, Wang Q, Yang L, Ji M, Xie D, Zhu S, Chen Y, Zhou Y, Higgins GA, Wiley JW, Hou X, Lin R
iScience 2023 Jul 21;26(7):107137

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m(6) A Reader YTHDF1-Targeting Engineered Small Extracellular Vesicles for Gastric Cancer Therapy via Epigenetic and Immune Regulation.

You Q, Wang F, Du R, Pi J, Wang H, Huo Y, Liu J, Wang C, Yu J, Yang Y, Zhu L
Advanced materials (Deerfield Beach, Fla.) 2023 Feb;35(8):e2204910

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Inhibition of Pard3 promotes breast cancer metastasis via the USP28 mediated deubiquitination of Snail1.

Liu Z, Yu Y, Zhou S, Zhang X, Zhou Z
Heliyon 2023 Dec;9(12):e22599

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Loss of BAF (mSWI/SNF) chromatin-remodeling ATPase Brg1 causes multiple malformations of cortical development in mice.

Jin Y, Gao X, Lu M, Chen G, Yang X, Ren N, Song Y, Hou C, Li J, Liu Q, Gao J
Human molecular genetics 2022 Oct 10;31(20):3504-3520

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Par3 promotes breast cancer invasion and migration through pull tension and protein nanoparticle-induced osmotic pressure.

Hu Y, Xie Q, Chen S, Zhao W, Zhao X, Ruan Q, Zheng Z, Zhao H, Ma T, Guo J, Li L
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2022 Nov;155:113739

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Ameloblastin promotes polarization of ameloblast cell lines in a 3-D cell culture system.

Visakan G, Su J, Moradian-Oldak J
Matrix biology : journal of the International Society for Matrix Biology 2022 Jan;105:72-86

---

Polarity protein Par3 sensitizes breast cancer to paclitaxel by promoting cell cycle arrest.

Zhao Y, Peng H, Liang L, Li Y, Hu X, Wang B, Xu Y, Chen S
Breast cancer research and treatment 2022 Feb;192(1):75-87

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PARD3 gene variation as candidate cause of nonsyndromic cleft palate only.

Cui R, Chen D, Li N, Cai M, Wan T, Zhang X, Zhang M, Du S, Ou H, Jiao J, Jiang N, Zhao S, Song H, Song X, Ma D, Zhang J, Li S
Journal of cellular and molecular medicine 2022 Aug;26(15):4292-4304

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Effects of hydrogen peroxide on endothelial function in three-dimensional hydrogel vascular model and regulation mechanism of polar protein Par3.

Gao H, Wang B, Chen R, Jin Z, Ren L, Yang J, Wang W, Zheng N, Lin R
Biomedical materials (Bristol, England) 2022 Aug 17;17(5)

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EMX2-GPR156-Gαi reverses hair cell orientation in mechanosensory epithelia.

Kindt KS, Akturk A, Jarysta A, Day M, Beirl A, Flonard M, Tarchini B
Nature communications 2021 May 17;12(1):2861

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Par3/integrin β1 regulates embryo adhesion via changing endometrial luminal epithelium polarity†.

Peng J, Li X, Zhang Y, Hu J, Shang Y, Yin Y, Xiao Z
Biology of reproduction 2021 Jun 4;104(6):1228-1238

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Syndecan-4/PAR-3 signaling regulates focal adhesion dynamics in mesenchymal cells.

Valdivia A, Cárdenas A, Brenet M, Maldonado H, Kong M, Díaz J, Burridge K, Schneider P, San Martín A, García-Mata R, Quest AFG, Leyton L
Cell communication and signaling : CCS 2020 Aug 18;18(1):129

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Influence of Wilms' tumor suppressor gene WT1 on bovine Sertoli cells polarity and tight junctions via non-canonical WNT signaling pathway.

Wang X, Adegoke EO, Ma M, Huang F, Zhang H, Adeniran SO, Zheng P, Zhang G
Theriogenology 2019 Oct 15;138:84-93

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Lgl1 deficiency disrupts hippocampal development and impairs cognitive performance in mice.

Zhang T, Hou C, Zhang S, Liu S, Li Z, Gao J
Genes, brain, and behavior 2019 Nov;18(8):e12605

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Coupling of apical-basal polarity and planar cell polarity to interpret the Wnt signaling gradient in feather development.

Lin J, Yue Z
Development (Cambridge, England) 2018 Sep 14;145(17)

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AhR activation protects intestinal epithelial barrier function through regulation of Par-6.

Yu K, Ma Y, Zhang Z, Fan X, Li T, Li L, Xiao W, Cai Y, Sun L, Xu P, Yu M, Yang H
Journal of molecular histology 2018 Oct;49(5):449-458

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Perturbation of epithelial apicobasal polarity by rhomboid family-1 gene overexpression.

Peng XM, Gao S, Deng HT, Cai HX, Zhou Z, Xiang R, Zhang QZ, Li LY
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 Oct;32(10):5577-5586

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[Influence of dexamethasone on the cell polarity and PAR complex of the embryonic epithelial cells in the palate].

Li M, Bing S, Qian Z
Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology 2018 Feb 1;36(1):9-16

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Deletion of Brg1 causes abnormal hair cell planer polarity, hair cell anchorage, and scar formation in mouse cochlea.

Jin Y, Ren N, Li S, Fu X, Sun X, Men Y, Xu Z, Zhang J, Xie Y, Xia M, Gao J
Scientific reports 2016 Jun 3;6:27124

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Par-3 modulates intestinal epithelial barrier function through regulating intracellular trafficking of occludin and myosin light chain phosphorylation.

Yu M, Yang S, Qiu Y, Chen G, Wang W, Xu C, Cai W, Sun L, Xiao W, Yang H
Journal of gastroenterology 2015 Nov;50(11):1103-13

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Overexpression of EphB4 in the mammary epithelium shifts the differentiation pathway of progenitor cells and promotes branching activity and vascularization.

Kaenel P, Hahnewald S, Wotzkow C, Strange R, Andres AC
Development, growth & differentiation 2014 May;56(4):255-75

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Transgenic expression of constitutively active RAC1 disrupts mouse rod morphogenesis.

Song H, Bush RA, Vijayasarathy C, Fariss RN, Kjellstrom S, Sieving PA
Investigative ophthalmology & visual science 2014 Apr 25;55(4):2659-68

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Deregulated ephrin-B2 signaling in mammary epithelial cells alters the stem cell compartment and interferes with the epithelial differentiation pathway.

Kaenel P, Antonijevic M, Richter S, Küchler S, Sutter N, Wotzkow C, Strange R, Andres AC
International journal of oncology 2012 Feb;40(2):357-69

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

MCF7 cells were subjected to SDS PAGE followed by western blot with 11085-1-AP(PARD3 antibody) at dilution of 1:300

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-PARD3 (IP:11085-1-AP, 4ug; Detection:11085-1-AP 1:200) with MCF-7 cells lysate 2900ug.

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human colon cancer using 11085-1-AP(PARD3 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human colon cancer using 11085-1-AP(PARD3 antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue

Flow cytometry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The PARD3 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human PARD3. This antibody recognizes human,mouse,rat antigen. The PARD3 antibody has been validated for the following applications: ELISA, WB, IHC, IP, FC analysis.

Appendix

11085-1-AP.pdf

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