NBP1-52654

RFX1 antibody from Novus Biologicals

EF-C

Western blot

Supportive data in Antibodypedia

Immunoprecipitation

Supportive data in Antibodypedia

Immunohistochemistry

Supportive data in Antibodypedia

Provider product page for NBP1-52654

Antibody Data

Product number

NBP1-52654

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-52654, RRID:AB_11026875

Product name

Rabbit Polyclonal RFX1 Antibody

Provider product page

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

References

RFX1 and RFX3 Transcription Factors Interact with the D Sequence of Adeno-Associated Virus Inverted Terminal Repeat and Regulate AAV Transduction.
Julien L, Chassagne J, Peccate C, Lorain S, Piétri-Rouxel F, Danos O, Benkhelifa-Ziyyat S
Scientific reports 2018 Jan 9;8(1):210

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Dynamics of the Transcriptome during Human Spermatogenesis: Predicting the Potential Key Genes Regulating Male Gametes Generation.
Zhu Z, Li C, Yang S, Tian R, Wang J, Yuan Q, Dong H, He Z, Wang S, Li Z
Scientific reports 2016 Jan 12;6:19069

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RFX1 Antibody [NBP1-52654] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-RFX1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. RFX1 was also immunoprecipitated by rabbit anti-RFX1 antibodies NBP1-52652 and NBP1-52653, which recognize upstream epitopes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RFX1 Antibody [NBP1-52654] - Detection of Human RFX1 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFX1 antibody NBP1-52654 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RFX1 Antibody [NBP1-52654] - Detection of Human RFX1 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFX1 antibody NBP1-52654 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: RFX1 Antibody [NBP1-52654] - Detection of human RFX1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RFX1 antibody NBP1-52654 used for IP at 6 ug per reaction. RFX1 was also immunoprecipitated by another rabbit anti-RFX1 antibody. For blotting immunoprecipitated RFX1, NBP1-52654 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: RFX1 Antibody [NBP1-52654] - Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- RFX1 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: RFX1 Antibody [NBP1-52654] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RFX1 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).