Antibody data
RFX1 and RFX3 Transcription Factors Interact with the D Sequence of Adeno-Associated Virus Inverted Terminal Repeat and Regulate AAV Transduction.
Julien L, Chassagne J, Peccate C, Lorain S, PiƩtri-Rouxel F, Danos O, Benkhelifa-Ziyyat S
Scientific reports 2018 Jan 9;8(1):210
Dynamics of the Transcriptome during Human Spermatogenesis: Predicting the Potential Key Genes Regulating Male Gametes Generation.
Zhu Z, Li C, Yang S, Tian R, Wang J, Yuan Q, Dong H, He Z, Wang S, Li Z
Scientific reports 2016 Jan 12;6:19069
Supportive validation
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Novus Biologicals (provider)
- Main image

- Experimental details
- Western Blot: RFX1 Antibody [NBP1-52654] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-RFX1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. RFX1 was also immunoprecipitated by rabbit anti-RFX1 antibodies NBP1-52652 and NBP1-52653, which recognize upstream epitopes.
Supportive validation
- Submitted by
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Novus Biologicals (provider)
- Main image

- Experimental details
- Western Blot: RFX1 Antibody [NBP1-52654] - Detection of Human RFX1 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFX1 antibody NBP1-52654 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
-
Novus Biologicals (provider)
- Main image

- Experimental details
- Western Blot: RFX1 Antibody [NBP1-52654] - Detection of Human RFX1 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFX1 antibody NBP1-52654 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
-
Novus Biologicals (provider)
- Main image

- Experimental details
- Immunoprecipitation: RFX1 Antibody [NBP1-52654] - Detection of human RFX1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RFX1 antibody NBP1-52654 used for IP at 6 ug per reaction. RFX1 was also immunoprecipitated by another rabbit anti-RFX1 antibody. For blotting immunoprecipitated RFX1, NBP1-52654 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Supportive validation
- Submitted by
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Novus Biologicals (provider)
- Main image

- Experimental details
- Immunohistochemistry-Paraffin: RFX1 Antibody [NBP1-52654] - Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- RFX1 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
Supportive validation
- Submitted by
-
Novus Biologicals (provider)
- Main image

- Experimental details
- Immunohistochemistry-Paraffin: RFX1 Antibody [NBP1-52654] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RFX1 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).