- GTX101147 - GeneTex HMOX1 antibody

Submitted references Protective Effects and Mechanisms of N-Phenethyl Caffeamide from UVA-Induced Skin Damage in Human Epidermal Keratinocytes through Nrf2/HO-1 Regulation.

Chu Y, Wu PY, Chen CW, Lyu JL, Liu YJ, Wen KC, Lin CY, Kuo YH, Chiang HM
International journal of molecular sciences 2019 Jan 4;20(1)

High Doses of Bupleurum falcatum Partially Prevents Estrogen Deficiency-Induced Bone Loss With Anti-osteoclastogenic Activity Due to Enhanced iNOS/NO Signaling.

Yeom M, Kim EY, Kim JH, Jung HS, Sohn Y
Frontiers in pharmacology 2018;9:1314

Autophagy Ablation in Adipocytes Induces Insulin Resistance and Reveals Roles for Lipid Peroxide and Nrf2 Signaling in Adipose-Liver Crosstalk.

Cai J, Pires KM, Ferhat M, Chaurasia B, Buffolo MA, Smalling R, Sargsyan A, Atkinson DL, Summers SA, Graham TE, Boudina S
Cell reports 2018 Nov 13;25(7):1708-1717.e5

Trans-cinnamic acid attenuates UVA-induced photoaging through inhibition of AP-1 activation and induction of Nrf2-mediated antioxidant genes in human skin fibroblasts.

Hseu YC, Korivi M, Lin FY, Li ML, Lin RW, Wu JJ, Yang HL
Journal of dermatological science 2018 May;90(2):123-134

PSMD4 is a novel therapeutic target in chemoresistant colorectal cancer activated by cytoplasmic localization of Nrf2.

Cheng YM, Lin PL, Wu DW, Wang L, Huang CC, Lee H
Oncotarget 2018 May 29;9(41):26342-26352

Kaempferol protects against propacetamol-induced acute liver injury through CYP2E1 inactivation, UGT1A1 activation, and attenuation of oxidative stress, inflammation and apoptosis in mice.

Tsai MS, Wang YH, Lai YY, Tsou HK, Liou GG, Ko JL, Wang SH
Toxicology letters 2018 Jun 15;290:97-109

A new copper ionophore DPMQ protects cells against ultraviolet B irradiation by inhibiting the TRPV1 channel.

Huang KF, Ma KH, Hung YC, Lo LC, Lin KC, Liu PS, Hu MK, Chueh SH
Journal of cellular physiology 2018 Dec;233(12):9594-9610

Molecular mechanisms of the effects of the ethanolic extract of Muntingia calabura Linn. fruit on lipopolysaccharide-induced pro-inflammatory mediators in macrophages.

Lin JT, Chang YY, Chen YC, Shen BY, Yang DJ
Food & function 2017 Mar 22;8(3):1245-1253

Continued 26S proteasome dysfunction in mouse brain cortical neurons impairs autophagy and the Keap1-Nrf2 oxidative defence pathway.

Ugun-Klusek A, Tatham MH, Elkharaz J, Constantin-Teodosiu D, Lawler K, Mohamed H, Paine SM, Anderson G, John Mayer R, Lowe J, Ellen Billett E, Bedford L
Cell death & disease 2017 Jan 5;8(1):e2531

Galangin ameliorates cisplatin-induced nephrotoxicity by attenuating oxidative stress, inflammation and cell death in mice through inhibition of ERK and NF-kappaB signaling.

Huang YC, Tsai MS, Hsieh PC, Shih JH, Wang TS, Wang YC, Lin TH, Wang SH
Toxicology and applied pharmacology 2017 Aug 15;329:128-139

Sulforaphane Suppresses Hepatitis C Virus Replication by Up-Regulating Heme Oxygenase-1 Expression through PI3K/Nrf2 Pathway.

Yu JS, Chen WC, Tseng CK, Lin CK, Hsu YC, Chen YH, Lee JC
PloS one 2016;11(3):e0152236

Cytoplasmic localization of Nrf2 promotes colorectal cancer with more aggressive tumors via upregulation of PSMD4.

Lin PL, Chang JT, Wu DW, Huang CC, Lee H
Free radical biology & medicine 2016 Jun;95:121-32

Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells.

Xu YM, Wu DD, Zheng W, Yu FY, Yang F, Yao Y, Zhou Y, Ching YP, Lau AT
Oncotarget 2016 Feb 2;7(5):6146-58

Tolerance to ischemia reperfusion injury in a congenital heart disease model.

Asada D, Itoi T, Nakamura A, Hamaoka K
Pediatrics international : official journal of the Japan Pediatric Society 2016 Dec;58(12):1266-1273

Dermato-protective properties of ergothioneine through induction of Nrf2/ARE-mediated antioxidant genes in UVA-irradiated Human keratinocytes.

Hseu YC, Lo HW, Korivi M, Tsai YC, Tang MJ, Yang HL
Free radical biology & medicine 2015 Sep;86:102-17

Baicalein Decreases Hydrogen Peroxide-Induced Damage to NG108-15 Cells via Upregulation of Nrf2.

Yeh CH, Ma KH, Liu PS, Kuo JK, Chueh SH
Journal of cellular physiology 2015 Aug;230(8):1840-51

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Heme Oxygenase 1 antibody detects HMOX1 protein by Western blot analysis. A. 30 µg NIH-3T3 whole cell lysate/extract B. 30 µg JC whole cell lysate/extract C. 30 µg BCL-1 whole cell lysate/extract D. 30 µg C2C12 whole cell lysate/extract E. 30 µg Raw264.7 whole cell lysate/extract 12% SDS-PAGE Heme Oxygenase 1 antibody (GTX101147) dilution: 1:1000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ug of whole cell lysate) A: H1299 B: Hela 12% SDS PAGE GTX101147 diluted at 1:1000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein by Western blot analysis.A. 50 µg mouse brain lysate/extract12 % SDS-PAGEHeme Oxygenase 1 antibody (GTX101147) dilution: 1:1000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein by western blot analysis.A. 30 ?g IMR32 whole cell lysate/extractB. 30 ?g SK-N-AS whole cell lysate/extract12% SDS-PAGEHeme Oxygenase 1 antibody (GTX101147) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein by western blot analysis.A. 30 ?g BCL-1 whole cell lysate/extractB. 30 ?g Raw264.7 whole cell lysate/extractC. 30 ?g C2C12 whole cell lysate/extract12 % SDS-PAGEHeme Oxygenase 1 antibody (GTX101147) dilution: 1:1000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein by western blot analysis.A. 50 ?g mouse brain lysate/extract12% SDS-PAGEHeme Oxygenase 1 antibody (GTX101147) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted by 1:1000.
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein by western blot analysis. Un-treated (-) and treated (+, 500 ?M CoCl2 treatment for 24 hr) HeLa whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) at a dilution of 1:1000.
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Histone H3K9K14ac (acetyl Lys9/Lys14) antibody detects Histone H3K9K14ac (acetyl Lys9/Lys14) protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H3K9K14ac (acetyl Lys9/Lys14) antibody (GTX101147) diluted at a dilution of 1:1000.
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Untreated (¡V) and treated (+) HeLa whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Untreated (¡V) and treated (+) HeLa whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Untreated (¡V) and treated (+) HeLa whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Wild-type (WT) and Heme Oxygenase 1 knockout (KO) HeLa cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Untreated (¡V) and treated (+) HeLa whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:5000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Untreated (¡V) and treated (+) HeLa whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:5000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at nucleus and cytoplasm by immunofluorescent analysis.Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Heme Oxygenase 1 protein stained by Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500.Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500.Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at cytoplasm on human renal carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human renal carcinoma . Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Heme Oxygenase 1 antibody detects HMOX1 protein at cytoplasm in rat spleen by immunohistochemical analysis. Sample: Paraffin-embedded rat spleen. Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500.

GTX101147

Antibody data