Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF3169 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal HO-1/HMOX1/HSP32 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse and rat HO-1/HMOX1/HSP32 in Western blots.
- Reactivity
- Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Royal jelly attenuates cadmium-induced nephrotoxicity in male mice.
Paeoniflorin Attenuated Oxidative Stress in Rat COPD Model Induced by Cigarette Smoke.
Almeer RS, AlBasher GI, Alarifi S, Alkahtani S, Ali D, Abdel Moneim AE
Scientific reports 2019 Apr 9;9(1):5825
Scientific reports 2019 Apr 9;9(1):5825
Paeoniflorin Attenuated Oxidative Stress in Rat COPD Model Induced by Cigarette Smoke.
Lin J, Xu F, Wang G, Kong L, Luo Q, Lv Y, Liu J, Wei Y, Li L, Zhang H, Dong J
Evidence-based complementary and alternative medicine : eCAM 2016;2016:1698379
Evidence-based complementary and alternative medicine : eCAM 2016;2016:1698379
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse/Rat HO-1/HMOX1/HSP32 by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line, A20 mouse B cell lymphoma cell line, NRK rat normal kidney cells, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse/Rat HO-1/HMOX1/HSP32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3169) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HO-1/HMOX1/HSP32 at approximately 32 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.