Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [2]
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Validation data
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- Product number
- NBP2-59492-100ul - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal HO-1/HMOX1/HSP32 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HO-1/HMOX1/HSP32 Antibody (1D6) [NBP2-59492] - Lane 1: HeLa cell lysates, Lane 2: A549 cell lysates, Lane 3: 293T cell lysates, Lane 4: HepG2 cell lysates, Lane 5: TF-1 cell lysates
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: HO-1/HMOX1/HSP32 Antibody (1D6) [NBP2-59492] - Flow cytometry analysis of HMOX in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: HO-1/HMOX1/HSP32 Antibody (1D6) [NBP2-59492] - Analysis of HMOX in HeLa cells. The cell was stained with NBP2-59492 at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).