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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- Ab107173 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Heme Oxygenase 1 Mouse mAb
- Antibody type
- Monoclonal
- Description
- Mouse anti Human Heme Oxygenase 1 Antibody, Monoclonal (A4), could be used for WB, IHC and so on. Application: WB: 0.01-5µg/mL IHC: 5-30µg/mL Protein Function Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
AA 61-172- Antibody clone number
- A4
- Vial size
- 100_l,10_l,1ml,20_l,50_l
- Concentration
- Lot by Lot
- Storage
- Store at 4_ short term (1-2 weeks). Store at -20_ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Heme Oxygenase 1 Mouse mAb (Ab107173) - Western Blot All lanes: Heme Oxygenase 1 Mouse mAb (Ab107173) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution Predicted band size: 33 kDa Observed band size: 30 kDa Exposure time: 15.0 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Heme Oxygenase 1 Mouse mAb (Ab107173) - IHC DAB staining on IHC Samples: Human Kidney Tissue Primary Ab: 30 µg/mL Heme Oxygenase 1 Mouse mAb (Ab107173) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
