Ab181869

antibody from Aladdin Scientific

Targeting: HMOX1 bK286B10, HO-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

Ab181869

Provider

Aladdin Scientific

Product name

HO-1 Mouse mAb

Antibody type

Monoclonal

Description

ApplicationWB: 1/1,000 - 1/200,000; Predicted molecular weight: 32.8 KDaICC/IF: 1/200

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

CD02/4E2

Vial size

100_l,10_l,1ml,50_l

Concentration

1 mg/ml

Storage

Store at 4¡C short term (1-2 weeks). Store at -20¡C or -80¡C. Avoid freeze / thaw cycle.

Western blot

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

HO-1 Mouse mAb (Ab181869) - Western Blot All lanes: HO-1 Mouse Antibody (Ab181869) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution Predicted band size: 33 kDa Observed band size: 30 kDa Exposure time: 8.7 s 

Immunocytochemistry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

HO-1 Mouse mAb (Ab181869) - ICC/IF IF analysis of HO-1(Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with HO-1 Mouse mAb (Ab181869) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.