- PA5-27338 - Invitrogen Antibodies HMOX1 antibody

Submitted references Preventive Effect of Limosilactobacillus fermentum SCHY34 on Lead Acetate-Induced Neurological Damage in SD Rats.

Long X, Wu H, Zhou Y, Wan Y, Kan X, Gong J, Zhao X
Frontiers in nutrition 2022;9:852012

Chronic Hematuria Increases Chronic Kidney Injury and Epithelial-Mesenchymal Transition in 5/6 Nephrectomy Rats.

Xiao M, Medipally AK, Biederman L, Satoskar AA, Ivanov I, Rovin BH, Brodsky SV
Frontiers in medicine 2021;8:753506

α-Lipoic Acid Maintains Brain Glucose Metabolism via BDNF/TrkB/HIF-1α Signaling Pathway in P301S Mice.

Zhang YH, Yan XZ, Xu SF, Pang ZQ, Li LB, Yang Y, Fan YG, Wang Z, Yu X, Guo C, Ao Q
Frontiers in aging neuroscience 2020;12:262

Defense and protection mechanisms in lung exposed to asbestiform fiber: the role of macrophage migration inhibitory factor and heme oxygenase-1.

Loreto C, Caltabiano R, Graziano ACE, Castorina S, Lombardo C, Filetti V, Vitale E, Rapisarda G, Cardile V, Ledda C, Rapisarda V
European journal of histochemistry : EJH 2020 Apr 16;64(2)

Physalis peruviana L. inhibits ovalbumin‑induced airway inflammation by attenuating the activation of NF‑κB and inflammatory molecules.

Park HA, Kwon OK, Ryu HW, Min JH, Park MW, Park MH, Paik JH, Choi S, Paryanto I, Yuniato P, Oh SR, Ahn KS, Lee JW
International journal of molecular medicine 2019 Apr;43(4):1830-1838

D‑Pinitol alleviates cyclosporine A‑induced renal tubulointerstitial fibrosis via activating Sirt1 and Nrf2 antioxidant pathways.

Koh ES, Kim S, Kim M, Hong YA, Shin SJ, Park CW, Chang YS, Chung S, Kim HS
International journal of molecular medicine 2018 Apr;41(4):1826-1834

Hemophagocytosis-mediated keratinization in oral carcinoma in situ and squamous cell carcinoma: a possible histopathogenesis of keratin pearls.

Al-Eryani K, Cheng J, Abé T, Yamazaki M, Maruyama S, Tsuneki M, Essa A, Babkair H, Saku T
Journal of cellular physiology 2013 Oct;228(10):1977-88

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Heme Oxygenase 1 using A) 30 µg IMR32 whole cell lysate and B) 30 µg SK-N-AS whole cell lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a Heme Oxygenase 1 polyclonal antibody (Product # PA5-27338) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Heme Oxygenase 1 using Whole cell extracts (30 µg). Samples were loaded onto a 12% SDS-PAGE gel and probed with a Heme Oxygenase 1 polyclonal antibody (Product # PA5-27338) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Heme Oxygenase 1 using A) 30 µg BCL-1 whole cell lysate (B) 30 µg Raw264.7 whole cell lysate and C) 30 µg C2C12 whole cell lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a Heme Oxygenase 1 polyclonal antibody (Product # PA5-27338) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Heme Oxygenase 1 using 50 µg mouse brain lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a Heme Oxygenase 1 polyclonal antibody (Product # PA5-27338) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of HMOX1 was performed by separating 50 µg of mouse tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a HMOX1 Polyclonal Antibody (Product # PA5-27338) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of HMOX1 was performed by separating 30 µg of untreated (–) and treated (+) HeLa whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a HMOX1 Polyclonal Antibody (Product # PA5-27338) at a dilution of 1:5000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of HMOX1 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a HMOX1 Polyclonal Antibody (Product # PA5-27338) at a dilution of 1:1500 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using HMOX1 Polyclonal Antibody (Product # PA5-27338). Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMOX1 Polyclonal Antibody (Product # PA5-27338) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Sandwich ELISA detection of recombinant full-length Heme Oxygenase 1 protein using HMOX1 Monoclonal Antibody (GT17811) (Product # MA5-31558) as capture antibody at concentration of 5 µg/mL and HMOX1 Polyclonal Antibody (Product # PA5-27338) as detection antibody at concentration of 1 µg/mL. Rabbit IgG antibody (HRP) was diluted at 1:10,000 and used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of HMOX1 was performed in Mock and treated HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: HMOX1 Polyclonal Antibody (Product # PA5-27338) diluted at 1:2000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Paraffin) analysis of HMOX1 was performed in paraffin-embedded rat spleen tissue using HMOX1 Polyclonal Antibody (Product # PA5-27338) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HMOX1 Polyclonal Antibody detects Heme Oxygenase 1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Heme Oxygenase 1 stained by HMOX1 Polyclonal Antibody (Product # PA5-27338) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HMOX1 Polyclonal Antibody detects Heme Oxygenase 1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. Heme Oxygenase 1 stained by HMOX1 Polyclonal Antibody (Product # PA5-27338) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HMOX1 Polyclonal Antibody detects Heme Oxygenase 1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat spleen. Heme Oxygenase 1 stained by HMOX1 Polyclonal Antibody (Product # PA5-27338) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HMOX1 Polyclonal Antibody detects Heme Oxygenase 1 protein at cytoplasm on human renal carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human renal carcinoma. HMOX1 Polyclonal Antibody (Product # PA5-27338) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 alpha-Lipoic acid (LA) improved glucose metabolism deficiency in P301S mice. (A) Representative western blots showed the expression levels of glucose transporter 1 (GLUT1), GLUT3, GLUT4, hexokinase-1 (HK-1), and HK2. (B-F) Quantified results of GLUT1, GLUT3, GLUT4, HK1, and HK2 levels between wild type (WT) and P301S mice. beta-actin served as an internal loading control. (G) HK activity between WT and P301S mice. (H-L) Quantified results of the levels of GLUT1, GLUT3, GLUT4, HK-1, and HK2 among vehicle, LA 3 mg/kg, and 10 mg/kg groups. beta-actin served as an internal loading control. (M) HK activity among vehicles, LA 3 mg/kg, and 10 mg/kg groups. (N-Q) Representative western blots and quantified results of the levels of heme oxygenase-1 (HO-1), vascular endothelial growth factor (VEGF), and proliferator-activated receptor gamma coactivator 1-alpha (PGC-1alpha). beta-actin served as an internal loading control. (R-U) mRNA level of GLUT1, GLUT3, GLUT4, and VEGF. Values are represented as the means +- SEM ( n = 7). * p < 0.05, ** p < 0.01.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1. A1) MIF IHC section of non-exposed sheep (magnification 200x). A2) MIF IHC section of exposed sheep; the fibrotic interstitium showed a strong scattered immunoreaction; green arrow indicates the intra-parenchymal stroma around a bronchiolar structure, yellow arrow shows MIF macrophages immunodetection while red arrow indicates FE deposit fibers (magnification 200x). A3) MIF immunostaining software image analysis of panel A2, in which mainly a high immunostained area (red color) was detected (magnification 200x). B1) HO-1 IHC section of non-exposed sheep (magnification 200x). B2) HO-1 IHC section of exposed sheep; in the lung fibrotic tissue, a strong and widespread immunostaining was demonstrated throughout the interstitium (black arrow) and bronchiolar structures (red arrow) (magnification 200x). B3) HO-1 immunostaining software image analysis of panel B2, in which mainly a high immunostained area (red color) was detected (magnification 200x).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2. A) Higher magnification of MIF immunohistochemical expression in exposed sheep section (magnification 400x). B) Higher magnification of HO-1 immunohistochemical expression in exposed sheep section (magnification 400x).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 4. HO-1 and MIF protein levels in human primary lung fibroblasts unexposed or FE-exposed for 72 h. Representative immunoblotting of HO-1 and MIF expressions (A). Results of three independent immunoblots are represented as percentage of HO-1 (B) and MIF (C) proteins with respect to untreated cells (*P

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 Expression of heme oxygenase 1 in the kidney and protein carbonyl contents in the renal cortex in kidneys in 5/6 nephrectomy rats with and without warfarin treatment before and at 26 weeks after the surgery. (A) A representative image of the renal cortex from a control 5/6 nephrectomy rat stained an antibody to heme oxygenase 1 (HMOX1). Immunohistochemistry, Magnification 200x. (B) A representative image of the renal cortex from a warfarin-treated 5/6 nephrectomy rat stained an antibody to heme oxygenase 1 (HMOX1). Immunohistochemistry, magnification 200x. (C) Quantitative analysis of HMOX1-positive staining in the tubules, percentage ( n = 6 in 5/6NE control; n = 7 in 5/6NE + warfarin groups). (D) Protein carbonyls were measured in the renal cortex in the right nephrectomy kidney at the time of 5/6 nephrectomy surgery and in the remnant kidney 26 weeks after the surgery. *, p < 0.005 as compared to protein carbonyl contents prior to the surgery; #, p < 0.005 as compared to control 5/6 nephrectomy rats.

PA5-27338

Antibody data