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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- BML-HC3001-0100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#BML-HC3001-0100, RRID:AB_11177779
- Product name
- Heme oxygenase 1 polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100 μl
- Storage
- -20°C
- Handling
- Store unopened vial at -20¡C until required for use. Store diluted antibody at 2-4¡C and use within 1 month. Avoid freeze/thaw cycles.
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Supportive validation
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- Enzo Life Sciences (provider)
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- Experimental details
- Western blot analysis: HO-1 was detected using HO-1, pAb (Prod. no. BML-HC3001) (lanes 1, 3, 5 and 7), and HO-2 was detected using HO-2, pAb (Prod. no. BML-HC3002) (lanes 2, 4, 6 and 8). Both antibodies were used at a dilution of 1:1000. Luminograph (ECL, 15 sec. exposure, ref. FM020/054) showing HO-1 and HO-2 expression in lysates prepared from gIFN/LPS stimulated RAW264.7 mouse macrophage cells (lanes 1 and 2), HepG2 (lanes 3 and 4), rat testis (HP9312, lanes 5 and 6) and rat spleen (HP9313, lanes 7 and 8).
