A305-847A-T

antibody from Invitrogen Antibodies

Targeting: METTL14 KIAA1627

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A305-847A-T

Provider

Invitrogen Antibodies

Product name

METTL14 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

10 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human METTL14 by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, RKO, GaMG, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-METTL14 antibody (Product # A305-847A-T lot 2) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse METTL14 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-METTL14 antibody (Product # A305-847A-T lot 2) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse METTL14 by western blot. Samples: Whole cell lysate (15 µg) from Jurkat, RKO, GaMG, and mouse TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-METTL14 antibody A305-847A-M (lot A305-847A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human METTL14 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-METTL14 antibody (Product # A305-847A-T; lot 2) used for IP at 20 µL/mg lysate. METTL14 was also immunoprecipitated by a previous lot of this antibody (Product # A305-847A-T; lot 1) and a second antibody against a different epitope of METTL14 (Product # A305-848A-T). For blotting immunoprecipitated METTL14, Product # A305-847A-T was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.