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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-30046 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GNB1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: Jurkat, Raji, NCI-H929, Mouse brain. Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (96%), Dog (100%), Chicken (97%), Rhesus Monkey (100%), Bovine (100%), Guinea pig (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 1.32 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of GNB1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: GNB1 Polyclonal Antibody (Product # PA5-30046) diluted at 1:500. Red: FIS1, a mitochondria marker. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of GNB1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: GNB1 Polyclonal Antibody (Product # PA5-30046) diluted at 1:500. Red: FIS1, a mitochondria marker. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of GNB1 was performed in 293T whole cell extracts using 5 µg of GNB1 Polyclonal Antibody (Product # PA5-30046). Samples were transferred to a membrane and probed with GNB1 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of GNB1 was performed in paraffin-embedded rat testis tissue using GNB1 Polyclonal Antibody (Product # PA5-30046) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of GNB1 was performed in paraffin-embedded mouse testis tissue using GNB1 Polyclonal Antibody (Product # PA5-30046) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lung papillory adenocarcinoma, using GNB1 (Product # PA5-30046) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of GNB1 was performed in 293T whole cell extracts using 5 µg of GNB1 Polyclonal Antibody (Product # PA5-30046). Samples were transferred to a membrane and probed with GNB1 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
