PA5-30046

antibody from Invitrogen Antibodies

Targeting: GNB1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30046

Provider

Invitrogen Antibodies

Product name

GNB1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: Jurkat, Raji, NCI-H929, Mouse brain. Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (96%), Dog (100%), Chicken (97%), Rhesus Monkey (100%), Bovine (100%), Guinea pig (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.44 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of GNB1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: GNB1 Polyclonal Antibody (Product # PA5-30046) diluted at 1:500. Red: FIS1, a mitochondria marker. Blue: Hoechst 33342 staining.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of GNB1 was performed in 293T whole cell extracts using 5 µg of GNB1 Polyclonal Antibody (Product # PA5-30046). Samples were transferred to a membrane and probed with GNB1 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.