RZ1045

antibody from NSJ Bioreagents

Targeting: GNB1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

RZ1045

Provider

NSJ Bioreagents

Product name

Zebrafish Gnb1 Antibody / Gnb1b

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Zebrafish

Host

Rabbit

Vial size

100 ug

Concentration

Lyophilized

Storage

After reconstitution, the Zebrafish Gnb1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of Gnb1/1b protein using Zebrafish Gnb1 antibody and 1) zebrafish head, 2) whole female zebrafish, 3) whole male zebrafish and 4) zebrafish embryo tissue lysate. Expected molecular weight ~37 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE zebrafish eye tissue with Zebrafish Gnb1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE zebrafish embryo tissue with Zebrafish Gnb1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE zebrafish embryo tissue with Zebrafish Gnb1 antibody. HIER: steam section in pH8 EDTA buffer for 20 min.