PA5-65793

antibody from Invitrogen Antibodies

Targeting: SEC24D KIAA0755

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-65793

Provider

Invitrogen Antibodies

Product name

SEC24D Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: IFLLANGLHMF LWLGVSSPPE LIQGIFNVPS FAHINTDMTL LPEVGNPYSQ QLRMIMGIIQ QKRPYSMKLT IVKQREQP Highest antigen sequence identity to the following orthologs - mouse 85%, rat 84%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.08 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Cytisine is neuroprotective in female but not male 6-hydroxydopamine lesioned parkinsonian mice and acts in combination with 17-β-estradiol to inhibit apoptotic endoplasmic reticulum stress in dopaminergic neurons.

Zarate SM, Pandey G, Chilukuri S, Garcia JA, Cude B, Storey S, Salem NA, Bancroft EA, Hook M, Srinivasan R
Journal of neurochemistry 2021 May;157(3):710-726

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of SEC24D in human cell line A-431 shows localization to vesicles. Samples were probed using a SEC24D Polyclonal Antibody (Product # PA5-65793).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

6 FIGURE Cytisine up-regulates Sec24D-containing ER exit sites (Sec24D-ERES) in mouse primary cultured dopaminergic (DA) neurons. (a) Representative confocal images of primary mouse DA neuron cell bodies stained for endogenous tyrosine hydroxylase (TH) (in red) and Sec24D-ERES (in green) are shown. Merged images for a control and 200 nM cytisine-treated DA neurons are shown along with separate images for TH and Sec24D staining. White arrowheads point to Sec24D-ERES puncta. Note the larger and more numerous Sec24D-ERES puncta following cytisine exposure. Scale bar is 5 mum. (b) Bar graphs quantifying the number, area, and intensity of Sec24D-ERES puncta in untreated (control) and cytisine-treated primary mouse DA cultures. All p values are based on Mann-Whitney test and error bars are S.E.M. ; n = 2 independent cultures and 60 DA neurons per condition; each independent culture is from embryonic day 14 embryos derived from multiple timed pregnant mice