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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- PA5-31191 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SEC24D Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa membrane.
- Concentration
- 1.01 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SEC24D using 20 µg of HeLa cell membrane lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a SEC24D polyclonal antibody (Product # PA5-31191) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SEC24D was performed by separating 30 µg of Whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a SEC24D Polyclonal Antibody (Product # PA5-31191) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
