H00000053-M01

antibody from Abnova Corporation

Targeting: ACP2 LAP

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000053-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000053-M01, RRID:AB_425282

Product name

ACP2 monoclonal antibody (M01), clone M1-4A12

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant ACP2.

Antigen sequence

MAGKRSGWSRAALLQLLLGVNLVVMPPTQARSLRF
VTLLYRHGDRSPVKTYPKDPYQEEEWPQGFGQLTK
EGMLQHWELGQALRQRYHGFLNTSYHRQEVYVRST
DFDRTLMSAEANLAGLFPPNGMQRFNPNISWQPIP
VHTVPITEDRLLKFPLGPCPRYEQLQNETRQTPEY
QNESSRNAQFLDMVANETGLTDLTLETVWNVYDTL
FCEQTHGLRLPPWASPQTMQRLSRLKDFSFRFLFG
IYQQAEKARLQGGVLLAQIRKNLTLMATTSQLPKL
LVYSAHDTTLVALQMALDVYNGEQAPYASCHIFEL
YQEDSGNFSVEMYFRNESDKAPWPLSLPGCPHRCP
LQDFLRLTEPVVPKDWQQECQLASGPADTEVIVAL
AVCGSILFLLIVLLLTVLFRMQAQPPGYRHVADGE
DHA


Isotype

IgG

Antibody clone number

M1-4A12

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of ACP2 expression in transfected 293T cell line by ACP2 monoclonal antibody (M01), clone M1-4A12.Lane 1: ACP2 transfected lysate(48.3 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged ACP2 is 1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to ACP2 on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to ACP2 on formalin-fixed paraffin-embedded human lymphoma tissue. [antibody concentration 5 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol