20164-1-AP

antibody from Proteintech Group

Targeting: MIEF1 FLJ20232, L0R8F8, MiD51, SMCR7L

Provider product page for 20164-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Antibody data

Antibody Data
Antigen structure
References [68]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunoprecipitation [1]

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Product number: 20164-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#20164-1-AP, RRID:AB_10639522
Product name: SMCR7L/MID51 antibody
Antibody type: Polyclonal
Description: KD/KO validated SMCR7L/MID51 antibody (Cat. #20164-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

MIEF1 protein structure - 20164-1-AP shown in red.

Submitted references Fatty acyl-coenzyme A activates mitochondrial division through oligomerization of MiD49 and MiD51.

Liu A, Kage F, Abdulkareem AF, Aguirre-Huamani MP, Sapp G, Aydin H, Higgs HN
Nature cell biology 2024 May;26(5):731-744

Inhibition of Drp1- Fis1 interaction alleviates aberrant mitochondrial fragmentation and acute kidney injury.

Song Z, Xia Y, Shi L, Zha H, Huang J, Xiang X, Li H, Huang H, Yue R, Wang H, Zhu J
Cellular & molecular biology letters 2024 Mar 4;29(1):31

Triaging of α-helical proteins to the mitochondrial outer membrane by distinct chaperone machinery based on substrate topology.

Muthukumar G, Stevens TA, Inglis AJ, Esantsi TK, Saunders RA, Schulte F, Voorhees RM, Guna A, Weissman JS
Molecular cell 2024 Mar 21;84(6):1101-1119.e9

MTFP1 controls mitochondrial fusion to regulate inner membrane quality control and maintain mtDNA levels.

Tábara LC, Burr SP, Frison M, Chowdhury SR, Paupe V, Nie Y, Johnson M, Villar-Azpillaga J, Viegas F, Segawa M, Anand H, Petkevicius K, Chinnery PF, Prudent J
Cell 2024 Jul 11;187(14):3619-3637.e27

Mitochondrial dysfunction induced by HIF-1α under hypoxia contributes to the development of gastric mucosal lesions.

Xiao Y, Liu X, Xie K, Luo J, Zhang Y, Huang X, Luo J, Tan S
Clinical and translational medicine 2024 Apr;14(4):e1653

The mystery of phospho-Drp1 with four adaptors in cell cycle: when mitochondrial fission couples to cell fate decisions.

Wu NS, Ma IC, Lin YF, Ko HJ, Loh JK, Hong YR
Cell cycle (Georgetown, Tex.) 2023 Nov;22(21-22):2485-2503

Senomorphic effect of diphenyleneiodonium through AMPK/MFF/DRP1 mediated mitochondrial fission.

Liao KM, Chen CJ, Luo WJ, Hsu CW, Yu SL, Yang PC, Su KY
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023 Jun;162:114616

Ischemia/reperfusion-induced MiD51 upregulation recruits Drp1 to mitochondria and contributes to myocardial injury.

Gao T, Shi R, Liu Z, De D, Li R, Chen Y, Pei J, Ding M
Biochemical and biophysical research communications 2023 Jul 12;665:78-87

Ren J, Liu J, Zhang J, Hu X, Cui Y, Wei X, Ma Y, Li X, Zhao Y
International journal of molecular sciences 2023 Dec 23;25(1)

PARK15/FBXO7 is dispensable for PINK1/Parkin mitophagy in iNeurons and HeLa cell systems.

Kraus F, Goodall EA, Smith IR, Jiang Y, Paoli JC, Adolf F, Zhang J, Paulo JA, Schulman BA, Harper JW
EMBO reports 2023 Aug 3;24(8):e56399

AMPK-dependent phosphorylation of MTFR1L regulates mitochondrial morphology.

Tilokani L, Russell FM, Hamilton S, Virga DM, Segawa M, Paupe V, Gruszczyk AV, Protasoni M, Tabara LC, Johnson M, Anand H, Murphy MP, Hardie DG, Polleux F, Prudent J
Science advances 2022 Nov 11;8(45):eabo7956

Novel roles of RTN4 and CLIMP-63 in regulating mitochondrial structure, bioenergetics and apoptosis.

Carter RJ, Milani M, Beckett AJ, Liu S, Prior IA, Cohen GM, Varadarajan S
Cell death & disease 2022 May 4;13(5):436

Mitochondrial matrix-localized Src kinase regulates mitochondrial morphology.

Lurette O, Guedouari H, Morris JL, Martín-Jiménez R, Robichaud JP, Hamel-Côté G, Khan M, Dauphinee N, Pichaud N, Prudent J, Hebert-Chatelain E
Cellular and molecular life sciences : CMLS 2022 May 30;79(6):327

Myosin II proteins are required for organization of calcium-induced actin networks upstream of mitochondrial division.

Kage F, Vicente-Manzanares M, McEwan BC, Kettenbach AN, Higgs HN
Molecular biology of the cell 2022 Jun 1;33(7):ar63

Characterisation of cold-induced mitochondrial fission in porcine aortic endothelial cells.

Quiring L, Walter B, Lohaus N, Schwan D, Rech A, Dlugos A, Rauen U
Molecular medicine (Cambridge, Mass.) 2022 Jan 31;28(1):13

Cdk5 Promotes Mitochondrial Fission via Drp1 Phosphorylation at S616 in Chronic Ethanol Exposure-Induced Cognitive Impairment.

Liu D, Li J, Rong X, Li J, Peng Y, Shen Q
Molecular neurobiology 2022 Dec;59(12):7075-7094

Small-molecule suppression of calpastatin degradation reduces neuropathology in models of Huntington's disease.

Hu D, Sun X, Magpusao A, Fedorov Y, Thompson M, Wang B, Lundberg K, Adams DJ, Qi X
Nature communications 2021 Sep 6;12(1):5305

King WT, Axelrod CL, Zunica ERM, Noland RC, Davuluri G, Fujioka H, Tandler B, Pergola K, Hermann GE, Rogers RC, López-Domènech S, Dantas WS, Stadler K, Hoppel CL, Kirwan JP
The Journal of biological chemistry 2021 Oct;297(4):101196

Hypothermia promotes mitochondrial elongation In cardiac cells via inhibition of Drp1.

Taylor D, Germano J, Song Y, Hadj-Moussa H, Marek-Iannucci S, Dhanji R, Sin J, Czer LSC, Storey KB, Gottlieb RA
Cryobiology 2021 Oct;102:42-55

ALS/FTD mutations in UBQLN2 are linked to mitochondrial dysfunction through loss-of-function in mitochondrial protein import.

Lin BC, Phung TH, Higgins NR, Greenslade JE, Prado MA, Finley D, Karbowski M, Polster BM, Monteiro MJ
Human molecular genetics 2021 Jun 17;30(13):1230-1246

Sirtuin 5 depletion impairs mitochondrial function in human proximal tubular epithelial cells.

Haschler TN, Horsley H, Balys M, Anderson G, Taanman JW, Unwin RJ, Norman JT
Scientific reports 2021 Jul 30;11(1):15510

MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.

König T, Nolte H, Aaltonen MJ, Tatsuta T, Krols M, Stroh T, Langer T, McBride HM
Nature cell biology 2021 Dec;23(12):1271-1286

Lipids activate skeletal muscle mitochondrial fission and quality control networks to induce insulin resistance in humans.

Axelrod CL, Fealy CE, Erickson ML, Davuluri G, Fujioka H, Dantas WS, Huang E, Pergola K, Mey JT, King WT, Mulya A, Hsia D, Burguera B, Tandler B, Hoppel CL, Kirwan JP
Metabolism: clinical and experimental 2021 Aug;121:154803

Pharmacological inhibition of dynamin-related protein 1 attenuates skeletal muscle insulin resistance in obesity.

Kugler BA, Deng W, Duguay AL, Garcia JP, Anderson MC, Nguyen PD, Houmard JA, Zou K
Physiological reports 2021 Apr;9(7):e14808

Mst1 Knockout Alleviates Mitochondrial Fission and Mitigates Left Ventricular Remodeling in the Development of Diabetic Cardiomyopathy.

Feng X, Wang S, Yang X, Lin J, Man W, Dong Y, Zhang Y, Zhao Z, Wang H, Sun D
Frontiers in cell and developmental biology 2020;8:628842

MiD51 Is Important for Maintaining Mitochondrial Health in Pancreatic Islet and MIN6 Cells.

Schultz J, Warkus J, Wolke C, Waterstradt R, Baltrusch S
Frontiers in endocrinology 2020;11:232

Aberrant Mitochondrial Morphology and Function in the BTBR Mouse Model of Autism Is Improved by Two Weeks of Ketogenic Diet.

Ahn Y, Sabouny R, Villa BR, Yee NC, Mychasiuk R, Uddin GM, Rho JM, Shutt TE
International journal of molecular sciences 2020 May 5;21(9)

Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.

Nagashima S, Tábara LC, Tilokani L, Paupe V, Anand H, Pogson JH, Zunino R, McBride HM, Prudent J
Science (New York, N.Y.) 2020 Mar 20;367(6484):1366-1371

NME3 Regulates Mitochondria to Reduce ROS-Mediated Genome Instability.

Chen CW, Tsao N, Zhang W, Chang ZF
International journal of molecular sciences 2020 Jul 17;21(14)

The microRNAs miR-302b and miR-372 regulate mitochondrial metabolism via the SLC25A12 transporter, which controls MAVS-mediated antiviral innate immunity.

Yasukawa K, Kinoshita D, Yaku K, Nakagawa T, Koshiba T
The Journal of biological chemistry 2020 Jan 10;295(2):444-457

Oligodendroglial glycolytic stress triggers inflammasome activation and neuropathology in Alzheimer's disease.

Zhang X, Wang R, Hu D, Sun X, Fujioka H, Lundberg K, Chan ER, Wang Q, Xu R, Flanagan ME, Pieper AA, Qi X
Science advances 2020 Dec;6(49)

Mitochondrial Safeguard: a stress response that offsets extreme fusion and protects respiratory function via flickering-induced Oma1 activation.

Murata D, Yamada T, Tokuyama T, Arai K, Quirós PM, López-Otín C, Iijima M, Sesaki H
The EMBO journal 2020 Dec 15;39(24):e105074

Cardiac Mitochondrial PTEN-L determines cell fate between apoptosis and survival during chronic alcohol consumption.

Sivakumar A, Shanmugarajan S, Subbiah R, Balakrishnan R
Apoptosis : an international journal on programmed cell death 2020 Aug;25(7-8):590-604

Klf5 down-regulation induces vascular senescence through eIF5a depletion and mitochondrial fission.

Ma D, Zheng B, Liu HL, Zhao YB, Liu X, Zhang XH, Li Q, Shi WB, Suzuki T, Wen JK
PLoS biology 2020 Aug;18(8):e3000808

Perturbations in Mitochondrial Dynamics Are Closely Involved in the Progression of Alcoholic Liver Disease.

Palma E, Riva A, Moreno C, Odena G, Mudan S, Manyakin N, Miquel R, Degré D, Trepo E, Sancho-Bru P, Altamirano J, Caballeria J, Zamalloa A, Menon K, Heaton N, Williams R, Bataller R, Chokshi S
Alcoholism, clinical and experimental research 2020 Apr;44(4):856-865

An epigenetic increase in mitochondrial fission by MiD49 and MiD51 regulates the cell cycle in cancer: Diagnostic and therapeutic implications.

Dasgupta A, Chen KH, Wu D, Hoskin V, Mewburn J, Lima PDA, Parlow LRG, Hindmarch CCT, Martin A, Sykes EA, Tayade C, Lightbody ED, Madarnas Y, SenGupta SK, Elliott BE, Nicol CJB, Archer SL
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Apr;34(4):5106-5127

Huangqi-Danshen Decoction Ameliorates Adenine-Induced Chronic Kidney Disease by Modulating Mitochondrial Dynamics.

Liu X, Huang S, Wang F, Zheng L, Lu J, Chen J, Li S
Evidence-based complementary and alternative medicine : eCAM 2019;2019:9574045

STX17 dynamically regulated by Fis1 induces mitophagy via hierarchical macroautophagic mechanism.

Xian H, Yang Q, Xiao L, Shen HM, Liou YC
Nature communications 2019 May 3;10(1):2059

Dynamin-1-Like Protein Inhibition Drives Megamitochondria Formation as an Adaptive Response in Alcohol-Induced Hepatotoxicity.

Palma E, Ma X, Riva A, Iansante V, Dhawan A, Wang S, Ni HM, Sesaki H, Williams R, Ding WX, Chokshi S
The American journal of pathology 2019 Mar;189(3):580-589

ATAD3A oligomerization causes neurodegeneration by coupling mitochondrial fragmentation and bioenergetics defects.

Zhao Y, Sun X, Hu D, Prosdocimo DA, Hoppel C, Jain MK, Ramachandran R, Qi X
Nature communications 2019 Mar 26;10(1):1371

Apogossypol-mediated reorganisation of the endoplasmic reticulum antagonises mitochondrial fission and apoptosis.

Yedida G, Milani M, Cohen GM, Varadarajan S
Cell death & disease 2019 Jul 8;10(7):521

Loss of the mitochondrial i-AAA protease YME1L leads to ocular dysfunction and spinal axonopathy.

Sprenger HG, Wani G, Hesseling A, König T, Patron M, MacVicar T, Ahola S, Wai T, Barth E, Rugarli EI, Bergami M, Langer T
EMBO molecular medicine 2019 Jan;11(1)

Two separate functions of NME3 critical for cell survival underlie a neurodegenerative disorder.

Chen CW, Wang HL, Huang CW, Huang CY, Lim WK, Tu IC, Koorapati A, Hsieh ST, Kan HW, Tzeng SR, Liao JC, Chong WM, Naroditzky I, Kidron D, Eran A, Nijim Y, Sela E, Feldman HB, Kalfon L, Raveh-Barak H, Falik-Zaccai TC, Elpeleg O, Mandel H, Chang ZF
Proceedings of the National Academy of Sciences of the United States of America 2019 Jan 8;116(2):566-574

Andrographolide alleviates Parkinsonism in MPTP-PD mice via targeting mitochondrial fission mediated by dynamin-related protein 1.

Geng J, Liu W, Gao J, Jiang C, Fan T, Sun Y, Qin ZH, Xu Q, Guo W, Gao J
British journal of pharmacology 2019 Dec;176(23):4574-4591

Loss of MIEF1/MiD51 confers susceptibility to BAX-mediated cell death and PINK1-PRKN-dependent mitophagy.

Xian H, Liou YC
Autophagy 2019 Dec;15(12):2107-2125

Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.

Zhong X, Cui P, Cai Y, Wang L, He X, Long P, Lu K, Yan R, Zhang Y, Pan X, Zhao X, Li W, Zhang H, Zhou Q, Gao P
Cell metabolism 2019 Apr 2;29(4):979-992.e4

Biventricular Increases in Mitochondrial Fission Mediator (MiD51) and Proglycolytic Pyruvate Kinase (PKM2) Isoform in Experimental Group 2 Pulmonary Hypertension-Novel Mitochondrial Abnormalities.

Xiong PY, Tian L, Dunham-Snary KJ, Chen KH, Mewburn JD, Neuber-Hess M, Martin A, Dasgupta A, Potus F, Archer SL
Frontiers in cardiovascular medicine 2018;5:195

In Vivo Deletion of β-Cell Drp1 Impairs Insulin Secretion Without Affecting Islet Oxygen Consumption.

Hennings TG, Chopra DG, DeLeon ER, VanDeusen HR, Sesaki H, Merrins MJ, Ku GM
Endocrinology 2018 Sep 1;159(9):3245-3256

Sam50 Regulates PINK1-Parkin-Mediated Mitophagy by Controlling PINK1 Stability and Mitochondrial Morphology.

Jian F, Chen D, Chen L, Yan C, Lu B, Zhu Y, Chen S, Shi A, Chan DC, Song Z
Cell reports 2018 Jun 5;23(10):2989-3005

INF2-mediated actin polymerization at the ER stimulates mitochondrial calcium uptake, inner membrane constriction, and division.

Chakrabarti R, Ji WK, Stan RV, de Juan Sanz J, Ryan TA, Higgs HN
The Journal of cell biology 2018 Jan 2;217(1):251-268

The Protein Coded by a Short Open Reading Frame, Not by the Annotated Coding Sequence, Is the Main Gene Product of the Dual-Coding Gene MIEF1.

Delcourt V, Brunelle M, Roy AV, Jacques JF, Salzet M, Fournier I, Roucou X
Molecular & cellular proteomics : MCP 2018 Dec;17(12):2402-2411

Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.

Wang L, Zhang T, Wang L, Cai Y, Zhong X, He X, Hu L, Tian S, Wu M, Hui L, Zhang H, Gao P
The EMBO journal 2017 May 15;36(10):1330-1347

Rapid degradation of mutant SLC25A46 by the ubiquitin-proteasome system results in MFN1/2-mediated hyperfusion of mitochondria.

Steffen J, Vashisht AA, Wan J, Jen JC, Claypool SM, Wohlschlegel JA, Koehler CM
Molecular biology of the cell 2017 Mar 1;28(5):600-612

Low-level laser therapy for beta amyloid toxicity in rat hippocampus.

Lu Y, Wang R, Dong Y, Tucker D, Zhao N, Ahmed ME, Zhu L, Liu TC, Cohen RM, Zhang Q
Neurobiology of aging 2017 Jan;49:165-182

Sirtuin 5 protects mitochondria from fragmentation and degradation during starvation.

Guedouari H, Daigle T, Scorrano L, Hebert-Chatelain E
Biochimica et biophysica acta. Molecular cell research 2017 Jan;1864(1):169-176

The Keap1-Nrf2 Stress Response Pathway Promotes Mitochondrial Hyperfusion Through Degradation of the Mitochondrial Fission Protein Drp1.

Sabouny R, Fraunberger E, Geoffrion M, Ng AC, Baird SD, Screaton RA, Milne R, McBride HM, Shutt TE
Antioxidants & redox signaling 2017 Dec 20;27(18):1447-1459

Effects of a Sublethal and Transient Stress of the Endoplasmic Reticulum on the Mitochondrial Population.

Vannuvel K, Van Steenbrugge M, Demazy C, Ninane N, Fattaccioli A, Fransolet M, Renard P, Raes M, Arnould T
Journal of cellular physiology 2016 Sep;231(9):1913-31

Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization.

Li H, Ruan Y, Zhang K, Jian F, Hu C, Miao L, Gong L, Sun L, Zhang X, Chen S, Chen H, Liu D, Song Z
Cell death and differentiation 2016 Mar;23(3):380-92

Cooperative and independent roles of the Drp1 adaptors Mff, MiD49 and MiD51 in mitochondrial fission.

Osellame LD, Singh AP, Stroud DA, Palmer CS, Stojanovski D, Ramachandran R, Ryan MT
Journal of cell science 2016 Jun 1;129(11):2170-81

FUNDC1 regulates mitochondrial dynamics at the ER-mitochondrial contact site under hypoxic conditions.

Wu W, Lin C, Wu K, Jiang L, Wang X, Li W, Zhuang H, Zhang X, Chen H, Li S, Yang Y, Lu Y, Wang J, Zhu R, Zhang L, Sui S, Tan N, Zhao B, Zhang J, Li L, Feng D
The EMBO journal 2016 Jul 1;35(13):1368-84

Drp1, Mff, Fis1, and MiD51 are coordinated to mediate mitochondrial fission during UV irradiation-induced apoptosis.

Zhang Z, Liu L, Wu S, Xing D
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2016 Jan;30(1):466-76

Loss of OMA1 delays neurodegeneration by preventing stress-induced OPA1 processing in mitochondria.

Korwitz A, Merkwirth C, Richter-Dennerlein R, Tröder SE, Sprenger HG, Quirós PM, López-Otín C, Rugarli EI, Langer T
The Journal of cell biology 2016 Jan 18;212(2):157-66

Mitochondrial E3 ubiquitin ligase MARCH5 controls mitochondrial fission and cell sensitivity to stress-induced apoptosis through regulation of MiD49 protein.

Xu S, Cherok E, Das S, Li S, Roelofs BA, Ge SX, Polster BM, Boyman L, Lederer WJ, Wang C, Karbowski M
Molecular biology of the cell 2016 Jan 15;27(2):349-59

Drp1-dependent mitochondrial fission via MiD49/51 is essential for apoptotic cristae remodeling.

Otera H, Miyata N, Kuge O, Mihara K
The Journal of cell biology 2016 Feb 29;212(5):531-44

Structural and functional analysis of MiD51, a dynamin receptor required for mitochondrial fission.

Richter V, Palmer CS, Osellame LD, Singh AP, Elgass K, Stroud DA, Sesaki H, Kvansakul M, Ryan MT
The Journal of cell biology 2014 Feb 17;204(4):477-86

Loss of Yme1L perturbates mitochondrial dynamics.

Ruan Y, Li H, Zhang K, Jian F, Tang J, Song Z
Cell death & disease 2013 Oct 31;4(10):e896

Fis1, Mff, MiD49, and MiD51 mediate Drp1 recruitment in mitochondrial fission.

Losón OC, Song Z, Chen H, Chan DC
Molecular biology of the cell 2013 Mar;24(5):659-67

A novel Drp1 inhibitor diminishes aberrant mitochondrial fission and neurotoxicity.

Qi X, Qvit N, Su YC, Mochly-Rosen D
Journal of cell science 2013 Feb 1;126(Pt 3):789-802

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HT-1080 cells were subjected to SDS PAGE followed by western blot with 20164-1-AP(SMCR7L antibody) at dilution of 1:400
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using SMCR7L antibody 20164-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP Result of anti-SMCR7L (IP:20164-1-AP, 3ug; Detection:20164-1-AP 1:1000) with RAW 264.7 cells lysate 2400ug.
Sample type: cell line