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LearnA06887-1
antibody from Boster Biological Technology
Targeting: MIEF1
FLJ20232, L0R8F8, MiD51, SMCR7L
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- A06887-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-MIEF1 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for MIEF1 detection. Tested with WB, IHC-P, ICC/IF, FCM, Direct ELISA in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references miR-494-5p mediates the antioxidant activity of EPA by targeting the mitochondrial elongation factor 1 gene MIEF1 in HepG2 cells.
Lin Y, Wu X, Lin L, Mei Y, Zhou J, Chen C, Li J, Wu D, Liu J, Li G
The Journal of nutritional biochemistry 2023 May;115:109279
The Journal of nutritional biochemistry 2023 May;115:109279
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of MIEF1 using anti-MIEF1 antibody (A06887-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HT1080 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: human HEK293 whole cell lysates, Lane 6: human U-937 whole cell lysates, Lane 7: human HepG2 whole cell lysates, Lane 8: rat heart tissue lysates, Lane 9: rat kidney tissue lysates, Lane 10: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIEF1 antigen affinity purified polyclonal antibody (Catalog # A06887-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MIEF1 at approximately 51KD. The expected band size for MIEF1 is at 51KD.
- Additional image
