HPA028521

antibody from Atlas Antibodies

Targeting: CFAP74 C1orf222, FLJ45476, KIAA1751

Antibody data

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Validation data

Product number: HPA028521 - Provider product page
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA028521, RRID:AB_10601881
Product name: Anti-CFAP74
Antibody type: Polyclonal
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Antigen sequence: HLRDFIHVDFDPPGPLSAGMSCEVLVTFKPMINKD LEGNISFLAQTGEFSVPLKCSTKKCSLSLDKELID FGSYVVGETTSRTITLTN
Isotype: IgG
Vial size: 100 µl
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

CFAP74 protein structure - HPA028521 shown in red.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
Main image
Experimental details: Immunohistochemistry analysis in human fallopian tube and placenta tissues using Anti-CFAP74 antibody. Corresponding CFAP74 RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Independent antibody validation
Main image
Experimental details: Immunohistochemical staining of human cerebral cortex, colon, fallopian tube and testis using Anti-CFAP74 antibody HPA028521 (A) shows similar protein distribution across tissues to independent antibody HPA029274 (B).
Antibody #2 product nr: HPA029274
Antibody provider: Atlas Antibodies

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human fallopian tube shows positivity in cilia.

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human fallopian tube shows high expression.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human placenta shows low expression as expected.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human cerebral cortex using Anti-CFAP74 antibody HPA028521.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human testis using Anti-CFAP74 antibody HPA028521.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human colon using Anti-CFAP74 antibody HPA028521.
Sample type: HUMAN