15277-1-AP
antibody from Proteintech Group
Targeting: MRPS25
DKFZp313H0817, FLJ00023, MRP-S25, RPMS25
Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
Reference
Comment
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- Product number
- 15277-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#15277-1-AP, RRID:AB_2180358
- Product name
- MRPS25 antibody
- Antibody type
- Polyclonal
- Description
- MRPS25 antibody (Cat. #15277-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references BOLA3 and NFU1 link mitoribosome iron-sulfur cluster assembly to multiple mitochondrial dysfunctions syndrome.
Linear Density Sucrose Gradients to Study Mitoribosomal Biogenesis in Tissue-Specific Knockout Mice.
Mutation in MRPS34 compromises protein synthesis and causes mitochondrial dysfunction.
Mutations in mitochondrial ribosomal protein MRPL12 leads to growth retardation, neurological deterioration and mitochondrial translation deficiency.
Human ERAL1 is a mitochondrial RNA chaperone involved in the assembly of the 28S small mitochondrial ribosomal subunit.
Zhong H, Janer A, Khalimonchuk O, Antonicka H, Shoubridge EA, Barrientos A
Nucleic acids research 2023 Nov 27;51(21):11797-11812
Nucleic acids research 2023 Nov 27;51(21):11797-11812
Linear Density Sucrose Gradients to Study Mitoribosomal Biogenesis in Tissue-Specific Knockout Mice.
Ruzzenente B, Metodiev MD
Methods in molecular biology (Clifton, N.J.) 2021;2224:47-60
Methods in molecular biology (Clifton, N.J.) 2021;2224:47-60
Mutation in MRPS34 compromises protein synthesis and causes mitochondrial dysfunction.
Richman TR, Ermer JA, Davies SM, Perks KL, Viola HM, Shearwood AM, Hool LC, Rackham O, Filipovska A
PLoS genetics 2015 Mar;11(3):e1005089
PLoS genetics 2015 Mar;11(3):e1005089
Mutations in mitochondrial ribosomal protein MRPL12 leads to growth retardation, neurological deterioration and mitochondrial translation deficiency.
Serre V, Rozanska A, Beinat M, Chretien D, Boddaert N, Munnich A, Rötig A, Chrzanowska-Lightowlers ZM
Biochimica et biophysica acta 2013 Aug;1832(8):1304-12
Biochimica et biophysica acta 2013 Aug;1832(8):1304-12
Human ERAL1 is a mitochondrial RNA chaperone involved in the assembly of the 28S small mitochondrial ribosomal subunit.
Dennerlein S, Rozanska A, Wydro M, Chrzanowska-Lightowlers ZM, Lightowlers RN
The Biochemical journal 2010 Sep 15;430(3):551-8
The Biochemical journal 2010 Sep 15;430(3):551-8
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 15277-1-AP(MRPS25 antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using MRPS25 antibody 15277-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line