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LearnOAAB06451
antibody from Aviva Systems Biology
Targeting: MRPS25
DKFZp313H0817, FLJ00023, MRP-S25, RPMS25
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- OAAB06451 - Provider product page
- Provider
- Aviva Systems Biology
- Product name
- MRPS25 antibody - C-terminal region
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400ul
- Storage
- Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of RT25 Antibody (C-term) in MCF-7 cell lysates (35ug/lane). RT25 (arrow) was detected using the purified Pab.
- Sample type
- MCF-7 cell line lysates
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- RT25 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RT25 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
- Sample type
- human lung tissue
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- RT25 Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Sample type
- MCF-7 cells
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
