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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- MA5-15036 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Pan-cadherin Monoclonal Antibody (R.976.9)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- R.976.9
- Vial size
- 100 µL
- Storage
- -20°C
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot was performed using Anti-Pan-cadherin Monoclonal Antibody (R.976.9) (Product # MA5-15036) and a 110kDa and 55kDa band corresponding to Cadherin was observed across in all the tested cell lines and tissues. Whole cell extracts (30 µg lysate) of HEL 92.1.7 (Lane 1), Caco-2 (Lane 2), Mouse Placenta (Lane 3), Rat Ovary (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
