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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [2]
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- Product number
- PA5-92365 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RRM2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Samples: MCF-7, SW480, HeLa, Jurkat, Mouse thymus; Cellular Location: Cytoplasm Immunogen sequence: MLSLRVPLAP ITDPQQLQLS PLKGLSLVDK ENTPPALSGT RVLASKTARR IFQEPTEPKT KAAAPGVEDE PLLRENPRRF VIFPIEYHDI WQMYKKAEAS FWTAEEVDLS KDIQHWESLK PEERYFISHV LAFFAASDGI VNENLVERFS QEVQITEARC FYGFQIAMEN IHSEMYSLLI DTYIKDPKER EFLFNAIETM PCVKKKADWA LRWIGDKEAT YGERVVAFAA VEGIFFSGSF ASIFWLKKRG LMPGLTFSNE LISRDEGLHC DFACLMFKHL VHKPSEERVR EIIINAVRIE QEFLTEALPV KLIGMNCTLM KQYIEFVADR LMLELGFSKV FRVENPFDFM ENISLEGKTN FFEKRVGEYQ RMGVMSSPTE NSFTLDADF
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.51 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Yap is essential for uterine decidualization through Rrm2/GSH/ROS pathway in response to Bmp2.
Yu HF, Yang ZQ, Xu MY, Huang JC, Yue ZP, Guo B
International journal of biological sciences 2022;18(6):2261-2276
International journal of biological sciences 2022;18(6):2261-2276
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of RRM2 was performed in A549 cells using RRM2 Polyclonal Antibody (Product # PA5-92365). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RRM2 in paraffin-embedded human colon carcinoma. Samples were incubated with RRM2 Polyclonal antibody (Product # PA5-92365) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RRM2 in paraffin-embedded human lung cancer. Samples were incubated with RRM2 Polyclonal antibody (Product # PA5-92365) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of RRM2 was performed in 200 µg extracts of HeLa cells using RRM2 Polyclonal Antibody (Product # PA5-92365). Western blot was performed from the immunoprecipitate using RRM2 Polyclonal Antibody at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rrm2 was direct target of Yap function in stromal differentiation and its expression was regulated by Bmp2 dependent on Yap. (A) Immunofluorescence analysis of Rrm2 expression in decidual cells (N = 3 per group). Scale bar, 60 um. (B and C) Rrm2 mRNA and protein expression after treatment with Yap siRNA or Verteporfin (N = 3 per group). (D) Schematic diagram exhibited Yap/Tead binding sites and mutation in Rrm2 promoter region. (E) Relative luciferase activity after pGL6-Rrm2 or pGL6-Rrm2-mutant plasmid was co-transfected with Yap siRNA (N = 6 per group). (F) Relative luciferase activity after introduction of pGL6-Rrm2 or pGL6-Rrm2-mutant plasmid along with an addition of Yap inhibitor Verteporfin (N = 6 per group). (G-J) Overexpression of Rrm2 rescued the defect of Prl8a2 and Prl3c1 expression as well as ALP activity elicited by Yap inactivation (N = 5 per group). (K-N) Blockage of Yap abrogated the regulation of Bmp2 on Rrm2 mRNA and protein expression (N = 3 per group).
