PA5-59813
antibody from Invitrogen Antibodies
Targeting: SPNS1
HSpin1, LAT, nrs, PP2030, SLC62A1, SPIN1, SPINL
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [12]
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Validation data
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- Product number
- PA5-59813 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SPNS1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MAGSDTAPFL SQADDPDDGP VPGTPGLPGS TGNPKSEEPE VPDQEGLQRI TGLSPGRS
- Concentration
- 0.1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SPNS1 in Lane 1: Marker (kDa) 250, 130, 100, 70, 55, 35, 25, 15, 10; Lane 2: Human cell line HEK 293. Samples were probed using a SPNS1 Polyclonal Antibody (Product # PA5-59813).
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SPNS1 in control (vector only transfected HEK293T lysate) and SPNS1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a SPNS1 Polyclonal Antibody (Product # PA5-59813).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SPNS1 in HEK293. Samples were incubated in SPNS1 polyclonal antibody (Product # PA5-59813).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SPNS1 in human parathyroid gland using SPNS1 Polyclonal Antibody (Product # PA5-59813) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical staining of SPNS1 in human pancreas using SPNS1 Polyclonal Antibody (Product # PA5-59813) shows low expression as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SPNS1 in human placenta using SPNS1 Polyclonal Antibody (Product # PA5-59813).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SPNS1 in human testis using SPNS1 Polyclonal Antibody (Product # PA5-59813).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SPNS1 in human liver using SPNS1 Polyclonal Antibody (Product # PA5-59813).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SPNS1 in human colon using SPNS1 Polyclonal Antibody (Product # PA5-59813).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SPNS1 in human lung and pancreas tissues. Samples were incubated in SPNS1 polyclonal antibody (Product # PA5-59813). Corresponding SPNS1 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SPNS1 in human lung, pancreas, placenta, and testis. Samples were incubated with SPSN1 polyclonal antibody (Product # PA5-59813). (A) shows similar protein distribution across tissues to (B) independent antibody (Product # PA5-60188).
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemistry analysis of SPNS1 in human placenta. Samples were incubated with SPNS1 polyclonal antibody (Product # PA5-59813). Shows strong granular cytoplasmic positivity in trophoblastic cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SPNS1 in human lung. Samples were incubated in SPNS1 polyclonal antibody (Product # PA5-59813). Shows strong granular cytoplasmic positivity in macrophages.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SPNS1 in human testis. Samples were incubated in SPNS1 polyclonal antibody (Product # PA5-59813). Shows weak granular cytoplasmic positivity in Leydig cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SPNS1 in human testis. Samples were incubated in SPNS1 polyclonal antibody (Product # PA5-59813). Shows very weak positivity in exocrine glandular cells as expected.