LS-C775955

antibody from LSBio

Targeting: REEP2 C5orf19, SGC32445, SPG72, Yip2d

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

LS-C775955

Provider

LSBio

Product name

REEP2 Antibody (clone S326D-2, AP) LS-C775955

Antibody type

Monoclonal

Description

Protein G purified

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Alkaline Phosphatase

Isotype

IgG

Antibody clone number

S326D-2

Storage

Store at -20°C.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western Blot analysis of COS-REEP2-His showing detection of ~ 30 kDa REEP2 protein using Mouse Anti-REEP2 Monoclonal Antibody, Clone S326D-2. Lane 1: MW Ladder. Lane 2: COS-REEP1-His. Lane 3: COS-REEP2-His. Lane 4: COS-REEP6-His. Load: 15 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-REEP2 Monoclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~ 30 kDa.

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-REEP2 Monoclonal Antibody, Clone S326D-2. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-REEP2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Mitochondrion, Mitochondrion Membrane, Endoplasmic Reticulum, Endoplasmic Reticulum Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) REEP2 Antibody. (D) Composite.