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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP11593PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP11593PU-N, RRID:AB_1753722
- Product name
- anti Hippocalcin-like protein 1 (HPCAL1) (N-term)
- Antibody type
- Polyclonal
- Antigen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-term region of human VILIP3.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of VILIP3 (arrow) using rabbit polyclonal VILIP3 Antibody (N-term) (AP11593PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the VILIP3 gene (Lane 2) (Origene Technologies).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- The anti-VILIP3 (AP11593PU-N) is used in Western blot to detect VILIP3 in mouse cerebellum tissue lysate.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinoma reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
