LS-C496731

antibody from LSBio

Targeting: HPCAL1 BDR1, HLP2, VILIP-3

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C496731

Provider

LSBio

Product name

HPCAL1 / Hippocalcin-Like 1 Antibody LS-C496731

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using HPCAL1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitation analysis of extracts of HepG2 cells using HPCAL1 antibody. Western blot was performed from the immunoprecipitate using HPCAL1 antibodyat a dilition of 1:1000.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation analysis of extracts of HepG2 cells using HPCAL1 antibody. Western blot was performed from the immunoprecipitate using HPCAL1 antibodyat a dilition of 1:1000.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation analysis of extracts of HepG2 cells using HPCAL1 antibody. Western blot was performed from the immunoprecipitate using HPCAL1 antibodyat a dilition of 1:1000.