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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB3605 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3605, RRID:AB_1576061
- Product name
- HPCAL1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of HPCAL1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Abnormal localization of two neuronal calcium sensor proteins, visinin-like proteins (vilips)-1 and -3, in neocortical brain areas of Alzheimer disease patients.
Regional and cellular distribution of neural visinin-like protein immunoreactivities (VILIP-1 and VILIP-3) in human brain.
Molecular cloning of a novel calcium-binding protein structurally related to hippocalcin from human brain and chromosomal mapping of its gene.
Braunewell K, Riederer P, Spilker C, Gundelfinger ED, Bogerts B, Bernstein HG
Dementia and geriatric cognitive disorders 2001 Mar-Apr;12(2):110-6
Dementia and geriatric cognitive disorders 2001 Mar-Apr;12(2):110-6
Regional and cellular distribution of neural visinin-like protein immunoreactivities (VILIP-1 and VILIP-3) in human brain.
Bernstein HG, Baumann B, Danos P, Diekmann S, Bogerts B, Gundelfinger ED, Braunewell KH
Journal of neurocytology 1999 Aug;28(8):655-62
Journal of neurocytology 1999 Aug;28(8):655-62
Molecular cloning of a novel calcium-binding protein structurally related to hippocalcin from human brain and chromosomal mapping of its gene.
Kobayashi M, Takamatsu K, Fujishiro M, Saitoh S, Noguchi T
Biochimica et biophysica acta 1994 Jul 21;1222(3):515-8
Biochimica et biophysica acta 1994 Jul 21;1222(3):515-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The HPCAL1 polyclonal antibody (Cat # PAB3605) is used in Western blot to detect HPCAL1 in mouse cerebellum tissue lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of HPCAL1 (arrow) using rabbit polyclonal HPCAL1 polyclonal antibody (Cat # PAB3605). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HPCAL1 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the HPCAL1 polyclonal antibody (Cat # PAB3605) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
