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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- PA5-27536 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HPCAL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HPCAL1 tansfected 293T cell, mouse brain. Predicted reactivity: Mouse (98%), Rat (98%), Zebrafish (90%), Xenopus laevis (96%), Pig (99%), Chicken (98%), Sheep (99%), Rhesus Monkey (99%), Chimpanzee (99%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.23 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of HPCAL1 was performed by separating of whole cell lysates by 12% SDS PAGE . Proteins were transferred to a membrane and probed with a HPCAL1 Polyclonal Antibody (Product # PA5-27536) at a dilution of 1:500. A: 293T cell line non-transfected lysate. , B: 293T cell line HPCAL1 transfected lysate. .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HPCAL1 Polyclonal Antibody (Product # PA5-27536). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 12% SDS PAGE. HPCAL1 Polyclonal Antibody (Product # PA5-27536) diluted at 1:1,000.
