Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-28784 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- eRF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: Raji. Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (100%), Dog (100%), Rabbit (100%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.8 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using eRF1 Polyclonal Antibody (Product # PA5-28784). Sample (30 µg of whole cell lysate). Lane A: Raji. 10% SDS PAGE. eRF1 Polyclonal Antibody . eRF1 Polyclonal Antibody (Product # PA5-28784) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of eRF1 in methanol-fixed HeLa cells using an ERF1 polyclonal antibody (Product # PA5-28784) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of eRF1 was performed in paraffin-embedded mouse brain tissue using eRF1 Polyclonal Antibody (Product # PA5-28784) at a dilution of 1:500.