Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [2]
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Validation data
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- Product number
- 17-9881-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HLA-DQ Monoclonal Antibody (SK10), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This SK10 monoclonal antibody reacts with the human MHC Class II HLA-DQ molecule. This cell surface receptor is a heterodimeric protein composed of an alpha (HLA-DQA1) and beta (HLA-DQB1) subunit. HLA-DQ is expressed on antigen presenting cells such as B cells, monocytes, macrophages, dendritic cells, and activated T cells. HLA-DQ plays a role in presenting peptide antigens to CD4+ T lymphocytes. Reports indicate that certain alleles of HLA-DQ are associated with celiac disease and type I diabetes. Applications Reported: This SK10 antibody has been reported for use in flow cytometric analysis. Applications Tested: This SK10 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- SK10
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Celiac Disease Defined by Over-Sensitivity to Gliadin Activation and Superior Antigen Presentation of Dendritic Cells.
Characteristic patterns of HLA presentation and T cell differentiation in adult-onset Still's disease.
A genetic perspective on coeliac disease.
A new automated human leukocyte antigen genotyping strategy to identify DR-DQ risk alleles for celiac disease and type 1 diabetes mellitus.
Activation pathways implicate anti-HLA-DP and anti-LFA-1 antibodies as lead candidates for intervention in chronic berylliosis.
Increased endothelial expression of HLA-DQ and interleukin 1alpha in extra-articular rheumatoid arthritis. Results from immunohistochemical studies of skeletal muscle.
Hudec M, Riegerová K, Pala J, Kútna V, Černá M, O Leary VB
International journal of molecular sciences 2021 Sep 15;22(18)
International journal of molecular sciences 2021 Sep 15;22(18)
Characteristic patterns of HLA presentation and T cell differentiation in adult-onset Still's disease.
Jung JY, Choi B, Sayeed HM, Suh CH, Kim YW, Kim HA, Sohn S
International journal of immunopathology and pharmacology 2018 Jan-Dec;32:2058738418791284
International journal of immunopathology and pharmacology 2018 Jan-Dec;32:2058738418791284
A genetic perspective on coeliac disease.
Trynka G, Wijmenga C, van Heel DA
Trends in molecular medicine 2010 Nov;16(11):537-50
Trends in molecular medicine 2010 Nov;16(11):537-50
A new automated human leukocyte antigen genotyping strategy to identify DR-DQ risk alleles for celiac disease and type 1 diabetes mellitus.
Lavant EH, Carlson JA
Clinical chemistry and laboratory medicine 2009;47(12):1489-95
Clinical chemistry and laboratory medicine 2009;47(12):1489-95
Activation pathways implicate anti-HLA-DP and anti-LFA-1 antibodies as lead candidates for intervention in chronic berylliosis.
Chou YK, Edwards DM, Weinberg AD, Vandenbark AA, Kotzin BL, Fontenot AP, Burrows GG
Journal of immunology (Baltimore, Md. : 1950) 2005 Apr 1;174(7):4316-24
Journal of immunology (Baltimore, Md. : 1950) 2005 Apr 1;174(7):4316-24
Increased endothelial expression of HLA-DQ and interleukin 1alpha in extra-articular rheumatoid arthritis. Results from immunohistochemical studies of skeletal muscle.
Turesson C, Englund P, Jacobsson LT, Sturfelt G, Truedsson L, Nennesmo I, Lundberg IE
Rheumatology (Oxford, England) 2001 Dec;40(12):1346-54
Rheumatology (Oxford, England) 2001 Dec;40(12):1346-54
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (blue histogram) or Anti-Human HLA-DQ APC (purple histogram). Cells in the lymphocyte gate were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Supplement Figure 1. Representative examples of flow cytometric histograms of surface-stained cells presenting human leukocyte antigen (HLA)-DQ from the peripheral blood of one patient with adult-onset Still's disease (AOSD), a patient with rheumatoid arthritis (RA), and a healthy control (HC).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1. Flow cytometric results: the percentage of surface-stained cells presenting HLA-DP, DR, and DQ in AOSD patients, RA patients, and HCs. Results were obtained from 14 patients with adult-onset Still's disease (AOSD), 20 with rheumatoid arthritis (RA), and 20 healthy controls (HC). The horizontal line indicates the mean value for each group. P values were determined by the Mann-Whitney U test.