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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Western blot [2]
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- Product number
- NB600-649-100ug - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Pipecolic acid oxidase Antibody
- Antibody type
- Polyclonal
- Description
- Ion exchange chromatography.
- Reactivity
- Bacteria
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Pipecolic acid oxidase Antibody [NB600-649] - Detection of Sarcosine Oxidase. Samples of 1 and 0.25 ug of purified Sarcosine Oxidase per lane were run by SDS-PAGE under reducing and non-reducing conditions and reduced samples contained 4% BME and were boiled for 5 minutes. Protein was transferred to nitrocellulose and probed with Goat anti Sarcosine Oxidase. Primary antibody was detected with Dylight 649 conjugated Donkey anti Goat (605-743-125 1:10K 1.5 hr RT in and imaged on the BioRad VersaDoc imaging system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Pipecolic acid oxidase Antibody [NB600-649] - Analysis using HRP conjugate of NB600-649. Lane 1: Sarcosine Oxidase (Microbial). Load: 50 ng per lane. Primary antibody: Goat anti-Sarcosine Oxidase (Microbial) Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 45 kDa, 45 kDa for Sarcosine Oxidase monomeric alpha subunit.
