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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- AF3204 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat RalA/RalB Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human, mouse, and rat RalA/RalB in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human H-Ras, N-Ras, or K-Ras is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P11234- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Expression of ral GTPases, their effectors, and activators in human bladder cancer.
Smith SC, Oxford G, Baras AS, Owens C, Havaleshko D, Brautigan DL, Safo MK, Theodorescu D
Clinical cancer research : an official journal of the American Association for Cancer Research 2007 Jul 1;13(13):3803-13
Clinical cancer research : an official journal of the American Association for Cancer Research 2007 Jul 1;13(13):3803-13
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human/Mouse/Rat RalA/RalB by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse/Rat RalA/RalB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3204) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human RalA and RalB (5 ng/lane) were included. A specific band for RalA/RalB was detected at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
