LS-C682371

antibody from LSBio

Targeting: RALA RAL

Western blot (Enhanced validation)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C682371

Provider

LSBio

Product name

RALA / RAL Antibody (clone 4G8C7) LS-C682371

Antibody type

Monoclonal

Description

Purified

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

4G8C7

Storage

Short term: store at 4°C. Long term: store at -20°C.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis using RALA mAb against HEK293 (1) and RALA (AA: 71-203)-hIgGFc transfected HEK293 (2) cell lysate.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis using RALA mAb against human RALA (AA: 71-203) recombinant protein. (Expected MW is 41.5 kDa)

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis using RALA mouse mAb against HepG2 (1), MCF-7 (2), A549 (3), K562 (4), Raji (5), MOLT4 (6), Hela (7), COS7 (8), and NIH3T3 (9) cell lysate.

ELISA

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Black line: Control Antigen (100 ng);Purple line: Antigen(10ng);Blue line: Antigen (50 ng);Red line: Antigen (100 ng);

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Black line: Control Antigen (100 ng);Purple line: Antigen(10ng);Blue line: Antigen (50 ng);Red line: Antigen (100 ng);

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Black line: Control Antigen (100 ng);Purple line: Antigen(10ng);Blue line: Antigen (50 ng);Red line: Antigen (100 ng);

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using RALA mouse mAb with DAB staining.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunofluorescence analysis of Hela cells using RALA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using RALA mouse mAb with DAB staining.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using RALA mouse mAb with DAB staining.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunofluorescence analysis of Hela cells using RALA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using RALA mouse mAb with DAB staining.