A301-458A

antibody from Invitrogen Antibodies

Targeting: CDK13 CDC2L, CDC2L5, CHED, KIAA1791

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A301-458A

Provider

Invitrogen Antibodies

Product name

CDC2L5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

The recommended shelf life for this product is 1 year from date of receipt.

Concentration

0.20 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CDC2L5 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, Jurkat, and KM12 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDC2L5 antibody (Product # A301-458A-2) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CDC2L5 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CDC2L5 antibody A301-458A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. CDC2L5 was also immunoprecipitated by rabbit anti-CDC2L5 antibody BL5851, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 3 minutes (A) and 10 seconds (B).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CDC2L5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CDC2L5 antibody (Product # A301-458A; Lot 2) used for IP at 6 µg per reaction. CDC2L5 was also immunoprecipitated by a previous lot of this antibody (Product # A301-458A; Lot 1) and a second antibody against a different epitope of CDC2L5 (BL5851). For blotting immunoprecipitated CDC2L5, Product # A301-458A was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.