Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- BS2649 - Provider product page
- Provider
- Bioworld Technology, Inc
- Proper citation
- Bioworld Technology Cat#BS2649, RRID:AB_1663756
- Product name
- PU.1 (R212) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide, corresponding to amino acids 180-230 of Human PU.1.
- Description
- The Ets transcription factor family (Ets-1, Ets-2, Erg-1ÿ3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1 and ERF) are DNA-binding proteins that influence lymphoid development and activity. The Ets family monomeric proteins bind the consensus DNA site GGA(A/T) through a unique winged helix-turn-helix motif known as the Ets domain. PU.1 (Spi-1/Spi-A), Spi-B and Spi-C are closely related Ets family members which share a conserved divergent sequence within the Ets domain that enables their binding to the non-canonical AGAA sites. PU.1 transactivates a large number of B cell genes, such as those encoding CD72, CD20 and Btk, and Spi-B enhances expression of many of these same target genes. PU.1 is expressed in a wide variety of hematopoetic cells, including B cells, early T cells, megakaryocytes, granulocytes, mast cells, immature erythrocytes and myeloid cells. Alternatively, Spi-B expression is limited to B cells and immature T cells, where expression accumulates through T lineage commitment and then is dramatically absent following the ÉÇ selection checkpoint.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of PU.1 (R212) pAb at 1:1000 dilution Lane1:JURKAT whole cell lysate(37ug) Lane2:THP-1 whole cell lysate(37ug) Lane3:The blood cell tissue lysate of Rat(36ug)